Transdermal delivery of (-)-epigallocatechin-3-gallate, a green tea polyphenol, in mice

Joshua D. Lambert, Dou Hwan Kim, Ruijin Zheng, Chung S. Yang

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Epigallocatechin-3-gallate (EGCG) is the most studied catechin in green tea (Camellia sinensis). EGCG and green tea are cancer preventive in many animal models, and numerous mechanisms have been proposed in cell lines. EGCG is poorly bioavailable in man and rodents. We hypothesized that transdermal delivery of EGCG could result in improved bioavailability. Following application of EGCG transdermal gel (50 mg kg-1, t.d.) to SKH-1 mice, EGCG was observed in the epidermis (1365.7-121.0 ng g-1) and dermis (411.2-42.6 ng g-1). The maximum plasma concentration (Cmax) of EGCG was 44.5 ng mL-1. The t1/2 (94.4 h) and AUC0→24h (881.5 ng mL-1 h) of EGCG were greater than values previously reported for oral EGCG. The t1/2 and area under the concentration-time curve up to 24h (AUC0→24h) in the liver, small intestine and colon were 21.3-74.6 h and 715-2802 ng g-1 h, respectively. Stability studies showed that the transdermal formulation was stable at 4°C and had a half-life (t1/2) of 47.1 and 20.2 h at 25°C and 37°C, respectively. These data indicate that transdermal EGCG is useful for delivering prolonged levels of EGCG to plasma and tissues, and may provide an alternative to tea consumption as a dosage form of EGCG.

Original languageEnglish (US)
Pages (from-to)599-604
Number of pages6
JournalJournal of Pharmacy and Pharmacology
Volume58
Issue number5
DOIs
StatePublished - May 1 2006

Fingerprint

Polyphenols
Tea
epigallocatechin gallate
Camellia sinensis
Catechin
Dosage Forms
Dermis
Epidermis
Biological Availability
Small Intestine
Half-Life
Rodentia
Colon
Animal Models
Gels

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

Lambert, Joshua D. ; Kim, Dou Hwan ; Zheng, Ruijin ; Yang, Chung S. / Transdermal delivery of (-)-epigallocatechin-3-gallate, a green tea polyphenol, in mice. In: Journal of Pharmacy and Pharmacology. 2006 ; Vol. 58, No. 5. pp. 599-604.
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Transdermal delivery of (-)-epigallocatechin-3-gallate, a green tea polyphenol, in mice. / Lambert, Joshua D.; Kim, Dou Hwan; Zheng, Ruijin; Yang, Chung S.

In: Journal of Pharmacy and Pharmacology, Vol. 58, No. 5, 01.05.2006, p. 599-604.

Research output: Contribution to journalArticle

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