@article{bcd1bc0bee144d0a99a5d0a8e523a4b7,
title = "Transfer functions linking neural calcium to single voxel functional ultrasound signal",
abstract = "Functional ultrasound imaging (fUS) is an emerging technique that detects changes of cerebral blood volume triggered by brain activation. Here, we investigate the extent to which fUS faithfully reports local neuronal activation by combining fUS and two-photon microscopy (2PM) in a co-registered single voxel brain volume. Using a machine-learning approach, we compute and validate transfer functions between dendritic calcium signals of specific neurons and vascular signals measured at both microscopic (2PM) and mesoscopic (fUS) levels. We find that transfer functions are robust across a wide range of stimulation paradigms and animals, and reveal a second vascular component of neurovascular coupling upon very strong stimulation. We propose that transfer functions can be considered as reliable quantitative reporters to follow neurovascular coupling dynamics.",
author = "Aydin, {Ali Kemal} and Haselden, {William D.} and {Goulam Houssen}, Yannick and Christophe Pouzat and Rungta, {Ravi L.} and Charlie Demen{\'e} and Mickael Tanter and Drew, {Patrick J.} and Serge Charpak and Davide Boido",
note = "Funding Information: Financial support was provided by the Institut National de la Sant{\'e} et de la Recherche M{\'e}dicale (INSERM), the European Research Council (ERC-2013-AD6; 339513), the Agence Nationale de la Recherche (ANR/NSF 15-NEUC-0003-02 and NR-16-RHUS-0004 [RHU TRT_cSVD]), the Fondation Leducq Transatlantic Networks of Excellence program (16CVD05, Understanding the role of the perivascular space in cerebral small vessel disease) and the IHU FOReSIGHT [ANR-18-IAHU-0001] supported by French state funds managed by the Agence Nationale de la Recherche within the Investissements d{\textquoteright}Avenir program. R.L.R. had a postdoctoral fellowship award from EMBO (ALTF 384-2015). We thank Manon Omnes for mice surgeries and Christophe Tourain for technical help. Publisher Copyright: {\textcopyright} 2020, The Author(s).",
year = "2020",
month = dec,
day = "1",
doi = "10.1038/s41467-020-16774-9",
language = "English (US)",
volume = "11",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",
}