TY - JOUR
T1 - Transformation in yeast
T2 - Development of a hybrid cloning vector and isolation of the can1 gene
AU - Broach, James R.
AU - Strathern, Jeffrey N.
AU - Hicks, James B.
N1 - Funding Information:
We wish to thank Carolyn McGill and Sajida Ismail for technical help and Louisa Dalessandro for preparation of the manuscript. This work was supported by National Institutes of Health General Medical Sciences grants GM24226 to J.R.B. and GM25624 to J.B.H. and J.N.D.
PY - 1979/12
Y1 - 1979/12
N2 - We have constructed a plasmid, YEp13, which when used in conjunction with transformation in yeast is a suitable vector for isolating specific yeast genes. The plasmid consists of pBR322, the LEU2 gene of yeast, and a DNA fragment containing a yeast origin of replication from 2μ circle. We have demonstrated the utility of this cloning system by isolating the yeast gene encoding the arginine permease, CAN1, from a pool of random yeast DNA fragments inserted into YEp13.
AB - We have constructed a plasmid, YEp13, which when used in conjunction with transformation in yeast is a suitable vector for isolating specific yeast genes. The plasmid consists of pBR322, the LEU2 gene of yeast, and a DNA fragment containing a yeast origin of replication from 2μ circle. We have demonstrated the utility of this cloning system by isolating the yeast gene encoding the arginine permease, CAN1, from a pool of random yeast DNA fragments inserted into YEp13.
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U2 - 10.1016/0378-1119(79)90012-X
DO - 10.1016/0378-1119(79)90012-X
M3 - Article
C2 - 395029
AN - SCOPUS:0018564346
VL - 8
SP - 121
EP - 133
JO - Gene
JF - Gene
SN - 0378-1119
IS - 1
ER -