Abstract
We have constructed a plasmid, YEp13, which when used in conjunction with transformation in yeast is a suitable vector for isolating specific yeast genes. The plasmid consists of pBR322, the LEU2 gene of yeast, and a DNA fragment containing a yeast origin of replication from 2μ circle. We have demonstrated the utility of this cloning system by isolating the yeast gene encoding the arginine permease, CAN1, from a pool of random yeast DNA fragments inserted into YEp13.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 121-133 |
| Number of pages | 13 |
| Journal | Gene |
| Volume | 8 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 1979 |
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All Science Journal Classification (ASJC) codes
- Genetics
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Transformation in yeast : Development of a hybrid cloning vector and isolation of the can1 gene. / Broach, James; Strathern, Jeffrey N.; Hicks, James B.
In: Gene, Vol. 8, No. 1, 01.01.1979, p. 121-133.Research output: Contribution to journal › Article
TY - JOUR
T1 - Transformation in yeast
T2 - Development of a hybrid cloning vector and isolation of the can1 gene
AU - Broach, James
AU - Strathern, Jeffrey N.
AU - Hicks, James B.
PY - 1979/1/1
Y1 - 1979/1/1
N2 - We have constructed a plasmid, YEp13, which when used in conjunction with transformation in yeast is a suitable vector for isolating specific yeast genes. The plasmid consists of pBR322, the LEU2 gene of yeast, and a DNA fragment containing a yeast origin of replication from 2μ circle. We have demonstrated the utility of this cloning system by isolating the yeast gene encoding the arginine permease, CAN1, from a pool of random yeast DNA fragments inserted into YEp13.
AB - We have constructed a plasmid, YEp13, which when used in conjunction with transformation in yeast is a suitable vector for isolating specific yeast genes. The plasmid consists of pBR322, the LEU2 gene of yeast, and a DNA fragment containing a yeast origin of replication from 2μ circle. We have demonstrated the utility of this cloning system by isolating the yeast gene encoding the arginine permease, CAN1, from a pool of random yeast DNA fragments inserted into YEp13.
UR - http://www.scopus.com/inward/record.url?scp=0018564346&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018564346&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(79)90012-X
DO - 10.1016/0378-1119(79)90012-X
M3 - Article
C2 - 395029
AN - SCOPUS:0018564346
VL - 8
SP - 121
EP - 133
JO - Gene
JF - Gene
SN - 0378-1119
IS - 1
ER -