Transformation in yeast: Development of a hybrid cloning vector and isolation of the can1 gene

James R. Broach, Jeffrey N. Strathern, James B. Hicks

Research output: Contribution to journalArticlepeer-review

660 Scopus citations

Abstract

We have constructed a plasmid, YEp13, which when used in conjunction with transformation in yeast is a suitable vector for isolating specific yeast genes. The plasmid consists of pBR322, the LEU2 gene of yeast, and a DNA fragment containing a yeast origin of replication from 2μ circle. We have demonstrated the utility of this cloning system by isolating the yeast gene encoding the arginine permease, CAN1, from a pool of random yeast DNA fragments inserted into YEp13.

Original languageEnglish (US)
Pages (from-to)121-133
Number of pages13
JournalGene
Volume8
Issue number1
DOIs
StatePublished - Dec 1979

All Science Journal Classification (ASJC) codes

  • Genetics

Fingerprint Dive into the research topics of 'Transformation in yeast: Development of a hybrid cloning vector and isolation of the can1 gene'. Together they form a unique fingerprint.

Cite this