Transformation in yeast: Development of a hybrid cloning vector and isolation of the can1 gene

James Broach, Jeffrey N. Strathern, James B. Hicks

Research output: Contribution to journalArticle

657 Citations (Scopus)

Abstract

We have constructed a plasmid, YEp13, which when used in conjunction with transformation in yeast is a suitable vector for isolating specific yeast genes. The plasmid consists of pBR322, the LEU2 gene of yeast, and a DNA fragment containing a yeast origin of replication from 2μ circle. We have demonstrated the utility of this cloning system by isolating the yeast gene encoding the arginine permease, CAN1, from a pool of random yeast DNA fragments inserted into YEp13.

Original languageEnglish (US)
Pages (from-to)121-133
Number of pages13
JournalGene
Volume8
Issue number1
DOIs
StatePublished - Jan 1 1979

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Genetic Vectors
Yeasts
Genes
Plasmids
Replication Origin
DNA
Organism Cloning

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Broach, James ; Strathern, Jeffrey N. ; Hicks, James B. / Transformation in yeast : Development of a hybrid cloning vector and isolation of the can1 gene. In: Gene. 1979 ; Vol. 8, No. 1. pp. 121-133.
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Transformation in yeast : Development of a hybrid cloning vector and isolation of the can1 gene. / Broach, James; Strathern, Jeffrey N.; Hicks, James B.

In: Gene, Vol. 8, No. 1, 01.01.1979, p. 121-133.

Research output: Contribution to journalArticle

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