Transformation of poplar by agrobacterium tumefaciens

Thomas John Parsons, Vilas P. Sinkar, Reinhard F. Stettler, Eugene W. Nester, Milton P. Gordon

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

The ability to regenerate plants from poplar cells cultured in vitro suggests that poplars may prove a valuable model system for the application of recombinant DNA technology to deciduous trees. We report here the transformation of a hybrid Populus trichocarpa X deltoides with two strains of Agrobacterium tumefaciens, as demonstrated by phytohormone autonomous growth, opine production, and Southern blot analysis.

Original languageEnglish (US)
Pages (from-to)533-536
Number of pages4
JournalBio/Technology
Volume4
Issue number6
DOIs
StatePublished - Jan 1 1986

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Populus
Genetic engineering
Agrobacterium tumefaciens
Plant Growth Regulators
Recombinant DNA
Plant Cells
Southern Blotting
Technology
Growth
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biotechnology

Cite this

Parsons, T. J., Sinkar, V. P., Stettler, R. F., Nester, E. W., & Gordon, M. P. (1986). Transformation of poplar by agrobacterium tumefaciens. Bio/Technology, 4(6), 533-536. https://doi.org/10.1038/nbt0686-533
Parsons, Thomas John ; Sinkar, Vilas P. ; Stettler, Reinhard F. ; Nester, Eugene W. ; Gordon, Milton P. / Transformation of poplar by agrobacterium tumefaciens. In: Bio/Technology. 1986 ; Vol. 4, No. 6. pp. 533-536.
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Parsons, TJ, Sinkar, VP, Stettler, RF, Nester, EW & Gordon, MP 1986, 'Transformation of poplar by agrobacterium tumefaciens', Bio/Technology, vol. 4, no. 6, pp. 533-536. https://doi.org/10.1038/nbt0686-533

Transformation of poplar by agrobacterium tumefaciens. / Parsons, Thomas John; Sinkar, Vilas P.; Stettler, Reinhard F.; Nester, Eugene W.; Gordon, Milton P.

In: Bio/Technology, Vol. 4, No. 6, 01.01.1986, p. 533-536.

Research output: Contribution to journalArticle

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Parsons TJ, Sinkar VP, Stettler RF, Nester EW, Gordon MP. Transformation of poplar by agrobacterium tumefaciens. Bio/Technology. 1986 Jan 1;4(6):533-536. https://doi.org/10.1038/nbt0686-533