Transient expression assay by agroinfiltration of leaves.

Min Woo Lee, Yinong Yang

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

An Agrobacterium-mediated transient expression assay has been described for in vivo analysis of constitutive or inducible gene expression in Arabidopsis plants. By simple infiltration of Agrobacterium cells carrying appropriate gene constructs into Arabidopsis leaves, transient expression assays can be performed within 3 d without using expensive instruments or complicated procedures. Two days after agroinfiltration, Arabidopsis plants can be treated with chemical inducers such as dexamethasone to induce transgene expression. The expression of the beta-glucoronidase (GUS) reporter gene can be determined by histochemical staining. In addition, RNA and protein can be extracted from agroinfected leaves and used for reverse transcription-polymerase chain reaction (RT-PCR), Northern blot, Western blot, immunoprecipitation, and enzyme assays.

Original languageEnglish (US)
Pages (from-to)225-229
Number of pages5
JournalMethods in molecular biology (Clifton, N.J.)
Volume323
StatePublished - Jan 1 2006

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Arabidopsis
Agrobacterium
Enzyme Assays
Transgenes
Reporter Genes
Immunoprecipitation
Northern Blotting
Dexamethasone
Reverse Transcription
Western Blotting
RNA
Staining and Labeling
Gene Expression
Polymerase Chain Reaction
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

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Transient expression assay by agroinfiltration of leaves. / Lee, Min Woo; Yang, Yinong.

In: Methods in molecular biology (Clifton, N.J.), Vol. 323, 01.01.2006, p. 225-229.

Research output: Contribution to journalArticle

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