Translational control of protein synthesis in muscle and liver of growth hormone-treated pigs

Jill A. Bush, Scot Kimball, Pamela M.J. O'Connor, Agus Suryawan, Renan A. Orellana, Hanh V. Nguyen, Leonard "Jim" Jefferson, Teresa A. Davis

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

GH treatment increases protein deposition and the efficiency of dietary protein used for growth. To identify the mechanisms that regulate tissue protein synthesis in response to exogenous GH treatment, fully fed, growing swine were treated with GH for 7 d. Fasted and fed pigs were infused with [1-13C]leucine to determine protein synthesis rates, and translation initiation factor activity levels were measured in skeletal muscle and liver. Feeding increased protein synthesis and translational efficiency in both muscle and liver of control and GH-treated pigs, and this was associated with increased 4E-BP1 and S6 kinase I phosphorylation, decreased association of eukaryotic initiation factor (eIF) 4E with 4E-BP1, and increased association of eIF4E with eIF4G. GH increased muscle protein synthesis and translational efficiency in fed pigs. GH increased liver protein synthesis of fasted and fed pigs in association with increased ribosome number. In muscle, but not liver, GH increased eIF2B activity and 4E-BP1 phosphorylation in both the fasted and fed state and increased the association of eIF4E with eIF4G in the fed state. We conclude that GH increases muscle protein synthesis in the fed state, in part, via mechanisms that enhance the binding of mRNA and methionyl-tRNA to the 40S ribosomal subunit, whereas GH increases liver protein synthesis in the fasted and fed states by increasing ribosome number. The results further indicate that the GH-induced protein synthetic response is dependent upon nutritional state and is tissue specific.

Original languageEnglish (US)
Pages (from-to)1273-1283
Number of pages11
JournalEndocrinology
Volume144
Issue number4
DOIs
StatePublished - Apr 1 2003

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Growth Hormone
Swine
Muscles
Liver
Proteins
Muscle Proteins
Ribosomes
Eukaryotic Small Ribosome Subunits
Phosphorylation
Eukaryotic Initiation Factor-4E
Ribosomal Protein S6 Kinases
Peptide Initiation Factors
Dietary Proteins
Transfer RNA
Leucine
Skeletal Muscle
Messenger RNA
Growth

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

Bush, J. A., Kimball, S., O'Connor, P. M. J., Suryawan, A., Orellana, R. A., Nguyen, H. V., ... Davis, T. A. (2003). Translational control of protein synthesis in muscle and liver of growth hormone-treated pigs. Endocrinology, 144(4), 1273-1283. https://doi.org/10.1210/en.2002-220983
Bush, Jill A. ; Kimball, Scot ; O'Connor, Pamela M.J. ; Suryawan, Agus ; Orellana, Renan A. ; Nguyen, Hanh V. ; Jefferson, Leonard "Jim" ; Davis, Teresa A. / Translational control of protein synthesis in muscle and liver of growth hormone-treated pigs. In: Endocrinology. 2003 ; Vol. 144, No. 4. pp. 1273-1283.
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Bush, JA, Kimball, S, O'Connor, PMJ, Suryawan, A, Orellana, RA, Nguyen, HV, Jefferson, LJ & Davis, TA 2003, 'Translational control of protein synthesis in muscle and liver of growth hormone-treated pigs', Endocrinology, vol. 144, no. 4, pp. 1273-1283. https://doi.org/10.1210/en.2002-220983

Translational control of protein synthesis in muscle and liver of growth hormone-treated pigs. / Bush, Jill A.; Kimball, Scot; O'Connor, Pamela M.J.; Suryawan, Agus; Orellana, Renan A.; Nguyen, Hanh V.; Jefferson, Leonard "Jim"; Davis, Teresa A.

In: Endocrinology, Vol. 144, No. 4, 01.04.2003, p. 1273-1283.

Research output: Contribution to journalArticle

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AU - Bush, Jill A.

AU - Kimball, Scot

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