Transscleral diffusion of ethacrynic acid and sodium fluorescein

Cheng Wen Lin, Yong Wang, Fan Yuan

Research output: Contribution to journalArticle

Abstract

Transscleral drug delivery is an effective method for treatment of eye diseases in posterior segment. Therefore, we investigated transscleral permeability of ethacrynic acid (ECA), a potential drug for treating glaucoma. To determine the ex vivo permeability of ECA across the sclera, we quantified the diffusion coefficients of two molecules, ECA and sodium fluorescein, in fresh porcine sclera mounted in a two-chamber diffusion apparatus. All experiments were conducted with phosphate-buffered solution (PBS) at 4°C for a period ranging from 2 to 14 hours. The concentrations of ECA and sodium fluorescein were measured at room temperature (25°C) with UV and fluorescence spectrophotometers, respectively. The results showed that the permeability of ECA (303 Da) was 4.21±0.82×10-5 cm/sec (n=9), whereas the permeability of sodium fluorescein (376 Da) was 1.06±0.88×10-6 cm/sec (n=12). These results suggest that the sclera tissue is more permeable to ECA than sodium fluorescein.

Original languageEnglish (US)
Pages (from-to)515
Number of pages1
JournalAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume1
StatePublished - 2002

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Ethacrynic Acid
Fluorescein
Sodium
Sclera
Acids
Permeability
Eye Diseases
Spectrophotometers
Drug delivery
Pharmaceutical Preparations
Glaucoma
Phosphates
Swine
Fluorescence
Tissue
Molecules
Temperature

All Science Journal Classification (ASJC) codes

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

Cite this

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title = "Transscleral diffusion of ethacrynic acid and sodium fluorescein",
abstract = "Transscleral drug delivery is an effective method for treatment of eye diseases in posterior segment. Therefore, we investigated transscleral permeability of ethacrynic acid (ECA), a potential drug for treating glaucoma. To determine the ex vivo permeability of ECA across the sclera, we quantified the diffusion coefficients of two molecules, ECA and sodium fluorescein, in fresh porcine sclera mounted in a two-chamber diffusion apparatus. All experiments were conducted with phosphate-buffered solution (PBS) at 4°C for a period ranging from 2 to 14 hours. The concentrations of ECA and sodium fluorescein were measured at room temperature (25°C) with UV and fluorescence spectrophotometers, respectively. The results showed that the permeability of ECA (303 Da) was 4.21±0.82×10-5 cm/sec (n=9), whereas the permeability of sodium fluorescein (376 Da) was 1.06±0.88×10-6 cm/sec (n=12). These results suggest that the sclera tissue is more permeable to ECA than sodium fluorescein.",
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T1 - Transscleral diffusion of ethacrynic acid and sodium fluorescein

AU - Lin, Cheng Wen

AU - Wang, Yong

AU - Yuan, Fan

PY - 2002

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AB - Transscleral drug delivery is an effective method for treatment of eye diseases in posterior segment. Therefore, we investigated transscleral permeability of ethacrynic acid (ECA), a potential drug for treating glaucoma. To determine the ex vivo permeability of ECA across the sclera, we quantified the diffusion coefficients of two molecules, ECA and sodium fluorescein, in fresh porcine sclera mounted in a two-chamber diffusion apparatus. All experiments were conducted with phosphate-buffered solution (PBS) at 4°C for a period ranging from 2 to 14 hours. The concentrations of ECA and sodium fluorescein were measured at room temperature (25°C) with UV and fluorescence spectrophotometers, respectively. The results showed that the permeability of ECA (303 Da) was 4.21±0.82×10-5 cm/sec (n=9), whereas the permeability of sodium fluorescein (376 Da) was 1.06±0.88×10-6 cm/sec (n=12). These results suggest that the sclera tissue is more permeable to ECA than sodium fluorescein.

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