traY and traI are required for oriT-dependent enhanced recombination between lac-containing plasmids and λplac5

J. R. Carter, R. D. Porter

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Recombination between F42lac and λplac5 is typically 20- to 50-fold more efficient than recombination between chromosomal lac and λplac5. This enhancement of recombination requires trans-acting factors located in the promoter-distal and promoter-proximal regions of the main traY-to-traI (traZ) operon. By testing the ability of deletion mutants of tra to support enhanced recombination, we have identified traY as the only essential gene from thepromoter-proximal region of tra. The possibility of a direct role for the traJ gene product has been ruled out. We also report that traI is the only gene from the promoter-distal end of the traY to traI operon that is required for recombination enhancement. Of the two proposed domains of traI, we conclude that the oriT-nicking activity is essential, whereas the helicase activity is largely dispensable. The possibility of a third traI activity is also discussed.

Original languageEnglish (US)
Pages (from-to)1027-1034
Number of pages8
JournalJournal of Bacteriology
Volume173
Issue number3
StatePublished - 1991

Fingerprint

Genetic Recombination
Plasmids
Operon
Trans-Activators
Essential Genes
Genetic Promoter Regions
Genes

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

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abstract = "Recombination between F42lac and λplac5 is typically 20- to 50-fold more efficient than recombination between chromosomal lac and λplac5. This enhancement of recombination requires trans-acting factors located in the promoter-distal and promoter-proximal regions of the main traY-to-traI (traZ) operon. By testing the ability of deletion mutants of tra to support enhanced recombination, we have identified traY as the only essential gene from thepromoter-proximal region of tra. The possibility of a direct role for the traJ gene product has been ruled out. We also report that traI is the only gene from the promoter-distal end of the traY to traI operon that is required for recombination enhancement. Of the two proposed domains of traI, we conclude that the oriT-nicking activity is essential, whereas the helicase activity is largely dispensable. The possibility of a third traI activity is also discussed.",
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traY and traI are required for oriT-dependent enhanced recombination between lac-containing plasmids and λplac5. / Carter, J. R.; Porter, R. D.

In: Journal of Bacteriology, Vol. 173, No. 3, 1991, p. 1027-1034.

Research output: Contribution to journalArticle

TY - JOUR

T1 - traY and traI are required for oriT-dependent enhanced recombination between lac-containing plasmids and λplac5

AU - Carter, J. R.

AU - Porter, R. D.

PY - 1991

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N2 - Recombination between F42lac and λplac5 is typically 20- to 50-fold more efficient than recombination between chromosomal lac and λplac5. This enhancement of recombination requires trans-acting factors located in the promoter-distal and promoter-proximal regions of the main traY-to-traI (traZ) operon. By testing the ability of deletion mutants of tra to support enhanced recombination, we have identified traY as the only essential gene from thepromoter-proximal region of tra. The possibility of a direct role for the traJ gene product has been ruled out. We also report that traI is the only gene from the promoter-distal end of the traY to traI operon that is required for recombination enhancement. Of the two proposed domains of traI, we conclude that the oriT-nicking activity is essential, whereas the helicase activity is largely dispensable. The possibility of a third traI activity is also discussed.

AB - Recombination between F42lac and λplac5 is typically 20- to 50-fold more efficient than recombination between chromosomal lac and λplac5. This enhancement of recombination requires trans-acting factors located in the promoter-distal and promoter-proximal regions of the main traY-to-traI (traZ) operon. By testing the ability of deletion mutants of tra to support enhanced recombination, we have identified traY as the only essential gene from thepromoter-proximal region of tra. The possibility of a direct role for the traJ gene product has been ruled out. We also report that traI is the only gene from the promoter-distal end of the traY to traI operon that is required for recombination enhancement. Of the two proposed domains of traI, we conclude that the oriT-nicking activity is essential, whereas the helicase activity is largely dispensable. The possibility of a third traI activity is also discussed.

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