Tri-layered elastomeric scaffolds for engineering heart valve leaflets

Nafiseh Masoumi, Nasim Annabi, Alexander Assmann, Benjamin L. Larson, Jesper Hjortnaes, Neslihan Alemdar, Mahshid Kharaziha, Keefe B. Manning, John E. Mayer, Ali Khademhosseini

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Tissue engineered heart valves (TEHVs) that can grow and remodel have the potential to serve as permanent replacements of the current non-viable prosthetic valves particularly for pediatric patients. A major challenge in designing functional TEHVs is to mimic both structural and anisotropic mechanical characteristics of the native valve leaflets. To establish a more biomimetic model of TEHV, we fabricated tri-layered scaffolds by combining electrospinning and microfabrication techniques. These constructs were fabricated by assembling microfabricated poly(glycerol sebacate) (PGS) and fibrous PGS/poly(caprolactone) (PCL) electrospun sheets to develop elastic scaffolds with tunable anisotropic mechanical properties similar to the mechanical characteristics of the native heart valves. The engineered scaffolds supported the growth of valvular interstitial cells (VICs) and mesenchymal stem cells (MSCs) within the 3D structure and promoted the deposition of heart valve extracellular matrix (ECM). MSCs were also organized and aligned along the anisotropic axes of the engineered tri-layered scaffolds. In addition, the fabricated constructs opened and closed properly in an exvivo model of porcine heart valve leaflet tissue replacement. The engineered tri-layered scaffolds have the potential for successful translation towards TEHV replacements.

Original languageEnglish (US)
Pages (from-to)7774-7785
Number of pages12
JournalBiomaterials
Volume35
Issue number27
DOIs
StatePublished - Sep 2014

Fingerprint

Heart Valves
Scaffolds
Tissue
Stem cells
Mesenchymal Stromal Cells
Microtechnology
Biomimetics
Pediatrics
Microfabrication
Electrospinning
Prosthetics
elastomeric
Glycerol
Extracellular Matrix
Swine
Cells
Mechanical properties
Growth

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Masoumi, N., Annabi, N., Assmann, A., Larson, B. L., Hjortnaes, J., Alemdar, N., ... Khademhosseini, A. (2014). Tri-layered elastomeric scaffolds for engineering heart valve leaflets. Biomaterials, 35(27), 7774-7785. https://doi.org/10.1016/j.biomaterials.2014.04.039
Masoumi, Nafiseh ; Annabi, Nasim ; Assmann, Alexander ; Larson, Benjamin L. ; Hjortnaes, Jesper ; Alemdar, Neslihan ; Kharaziha, Mahshid ; Manning, Keefe B. ; Mayer, John E. ; Khademhosseini, Ali. / Tri-layered elastomeric scaffolds for engineering heart valve leaflets. In: Biomaterials. 2014 ; Vol. 35, No. 27. pp. 7774-7785.
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Masoumi, N, Annabi, N, Assmann, A, Larson, BL, Hjortnaes, J, Alemdar, N, Kharaziha, M, Manning, KB, Mayer, JE & Khademhosseini, A 2014, 'Tri-layered elastomeric scaffolds for engineering heart valve leaflets', Biomaterials, vol. 35, no. 27, pp. 7774-7785. https://doi.org/10.1016/j.biomaterials.2014.04.039

Tri-layered elastomeric scaffolds for engineering heart valve leaflets. / Masoumi, Nafiseh; Annabi, Nasim; Assmann, Alexander; Larson, Benjamin L.; Hjortnaes, Jesper; Alemdar, Neslihan; Kharaziha, Mahshid; Manning, Keefe B.; Mayer, John E.; Khademhosseini, Ali.

In: Biomaterials, Vol. 35, No. 27, 09.2014, p. 7774-7785.

Research output: Contribution to journalArticle

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AU - Masoumi, Nafiseh

AU - Annabi, Nasim

AU - Assmann, Alexander

AU - Larson, Benjamin L.

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AU - Alemdar, Neslihan

AU - Kharaziha, Mahshid

AU - Manning, Keefe B.

AU - Mayer, John E.

AU - Khademhosseini, Ali

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Masoumi N, Annabi N, Assmann A, Larson BL, Hjortnaes J, Alemdar N et al. Tri-layered elastomeric scaffolds for engineering heart valve leaflets. Biomaterials. 2014 Sep;35(27):7774-7785. https://doi.org/10.1016/j.biomaterials.2014.04.039