Trichloroethylene degradation and mineralization by pseudomonads and Methylosinus trichosporium 0B3b

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

To examine the trichloroethylene (C2HCl3)-degrading capability of five microorganisms, the maximum rate, extent, and degree of C2HCl3 mineralization were evaluated for Pseudomonas cepacia G4, Pseudomonas cepacia G4 PR1, Pseudomonas mendocina KR1, Pseudomonas putida F1, and Methylosinus trichosporium OB3b using growth conditions commonly reported in the literature for expression of oxygenases responsible for C2HCl3 degradation. By varying the C2HCl3 concentration from 5 μM to 75 μM, Vmax and Km values for C2HCl3 degradation were calculated as 9 nmol/(min mg protein) and 4 μM for P. cepacia G4, 18 nmol/(min mg protein) and 29 μM for P. cepacia G4 PR1, 20 nmol/(min mg protein) and 10 μM for P. mendocina KR1, and 8 nmol/(min mg protein) and 5 μM for P. putida F1. This is the first report of these Michaelis-Menten parameters for P. mendocina KR1, P. putida F1, and P. cepacia G4 PR1. At 75 μM, the extent of C2HCl3 that was degraded after 6 h of incubation with resting cells was 61% 98%; the highest degradation being achieved by toluene-induced P. mendocina KR1. The extent of C2HCl3 mineralization in 6 h (as indicated by concentration of chloride ion) was also measured and varied from 36% for toluene-induced P. putida F1 to 102% for M. trichosporium OB3b. Since C2HCl3 degradation requires new bio-mass, the specific growth rate (μmax) of each of the C2HCl3-degradation microorganisms was determined and varied from 0.080/h (M. trichosporium OB3b) to 0.864/h (P. cepacia G4PR1).

Original languageEnglish (US)
Pages (from-to)248-256
Number of pages9
JournalApplied Microbiology and Biotechnology
Volume45
Issue number1-2
DOIs
StatePublished - Jan 1 1996

Fingerprint

Methylosinus trichosporium
Burkholderia cepacia
Trichloroethylene
Pseudomonas mendocina
Pseudomonas putida
Toluene
Proteins
Oxygenases
Growth
Chlorides
Ions

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

@article{f78c2f9075c64fb1bb2f32f24781acff,
title = "Trichloroethylene degradation and mineralization by pseudomonads and Methylosinus trichosporium 0B3b",
abstract = "To examine the trichloroethylene (C2HCl3)-degrading capability of five microorganisms, the maximum rate, extent, and degree of C2HCl3 mineralization were evaluated for Pseudomonas cepacia G4, Pseudomonas cepacia G4 PR1, Pseudomonas mendocina KR1, Pseudomonas putida F1, and Methylosinus trichosporium OB3b using growth conditions commonly reported in the literature for expression of oxygenases responsible for C2HCl3 degradation. By varying the C2HCl3 concentration from 5 μM to 75 μM, Vmax and Km values for C2HCl3 degradation were calculated as 9 nmol/(min mg protein) and 4 μM for P. cepacia G4, 18 nmol/(min mg protein) and 29 μM for P. cepacia G4 PR1, 20 nmol/(min mg protein) and 10 μM for P. mendocina KR1, and 8 nmol/(min mg protein) and 5 μM for P. putida F1. This is the first report of these Michaelis-Menten parameters for P. mendocina KR1, P. putida F1, and P. cepacia G4 PR1. At 75 μM, the extent of C2HCl3 that was degraded after 6 h of incubation with resting cells was 61{\%} 98{\%}; the highest degradation being achieved by toluene-induced P. mendocina KR1. The extent of C2HCl3 mineralization in 6 h (as indicated by concentration of chloride ion) was also measured and varied from 36{\%} for toluene-induced P. putida F1 to 102{\%} for M. trichosporium OB3b. Since C2HCl3 degradation requires new bio-mass, the specific growth rate (μmax) of each of the C2HCl3-degradation microorganisms was determined and varied from 0.080/h (M. trichosporium OB3b) to 0.864/h (P. cepacia G4PR1).",
author = "Sun, {A. K.} and Wood, {Thomas Keith}",
year = "1996",
month = "1",
day = "1",
doi = "10.1007/s002530050679",
language = "English (US)",
volume = "45",
pages = "248--256",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
publisher = "Springer Verlag",
number = "1-2",

}

Trichloroethylene degradation and mineralization by pseudomonads and Methylosinus trichosporium 0B3b. / Sun, A. K.; Wood, Thomas Keith.

In: Applied Microbiology and Biotechnology, Vol. 45, No. 1-2, 01.01.1996, p. 248-256.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Trichloroethylene degradation and mineralization by pseudomonads and Methylosinus trichosporium 0B3b

AU - Sun, A. K.

AU - Wood, Thomas Keith

PY - 1996/1/1

Y1 - 1996/1/1

N2 - To examine the trichloroethylene (C2HCl3)-degrading capability of five microorganisms, the maximum rate, extent, and degree of C2HCl3 mineralization were evaluated for Pseudomonas cepacia G4, Pseudomonas cepacia G4 PR1, Pseudomonas mendocina KR1, Pseudomonas putida F1, and Methylosinus trichosporium OB3b using growth conditions commonly reported in the literature for expression of oxygenases responsible for C2HCl3 degradation. By varying the C2HCl3 concentration from 5 μM to 75 μM, Vmax and Km values for C2HCl3 degradation were calculated as 9 nmol/(min mg protein) and 4 μM for P. cepacia G4, 18 nmol/(min mg protein) and 29 μM for P. cepacia G4 PR1, 20 nmol/(min mg protein) and 10 μM for P. mendocina KR1, and 8 nmol/(min mg protein) and 5 μM for P. putida F1. This is the first report of these Michaelis-Menten parameters for P. mendocina KR1, P. putida F1, and P. cepacia G4 PR1. At 75 μM, the extent of C2HCl3 that was degraded after 6 h of incubation with resting cells was 61% 98%; the highest degradation being achieved by toluene-induced P. mendocina KR1. The extent of C2HCl3 mineralization in 6 h (as indicated by concentration of chloride ion) was also measured and varied from 36% for toluene-induced P. putida F1 to 102% for M. trichosporium OB3b. Since C2HCl3 degradation requires new bio-mass, the specific growth rate (μmax) of each of the C2HCl3-degradation microorganisms was determined and varied from 0.080/h (M. trichosporium OB3b) to 0.864/h (P. cepacia G4PR1).

AB - To examine the trichloroethylene (C2HCl3)-degrading capability of five microorganisms, the maximum rate, extent, and degree of C2HCl3 mineralization were evaluated for Pseudomonas cepacia G4, Pseudomonas cepacia G4 PR1, Pseudomonas mendocina KR1, Pseudomonas putida F1, and Methylosinus trichosporium OB3b using growth conditions commonly reported in the literature for expression of oxygenases responsible for C2HCl3 degradation. By varying the C2HCl3 concentration from 5 μM to 75 μM, Vmax and Km values for C2HCl3 degradation were calculated as 9 nmol/(min mg protein) and 4 μM for P. cepacia G4, 18 nmol/(min mg protein) and 29 μM for P. cepacia G4 PR1, 20 nmol/(min mg protein) and 10 μM for P. mendocina KR1, and 8 nmol/(min mg protein) and 5 μM for P. putida F1. This is the first report of these Michaelis-Menten parameters for P. mendocina KR1, P. putida F1, and P. cepacia G4 PR1. At 75 μM, the extent of C2HCl3 that was degraded after 6 h of incubation with resting cells was 61% 98%; the highest degradation being achieved by toluene-induced P. mendocina KR1. The extent of C2HCl3 mineralization in 6 h (as indicated by concentration of chloride ion) was also measured and varied from 36% for toluene-induced P. putida F1 to 102% for M. trichosporium OB3b. Since C2HCl3 degradation requires new bio-mass, the specific growth rate (μmax) of each of the C2HCl3-degradation microorganisms was determined and varied from 0.080/h (M. trichosporium OB3b) to 0.864/h (P. cepacia G4PR1).

UR - http://www.scopus.com/inward/record.url?scp=0029938055&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029938055&partnerID=8YFLogxK

U2 - 10.1007/s002530050679

DO - 10.1007/s002530050679

M3 - Article

C2 - 8920197

AN - SCOPUS:0029938055

VL - 45

SP - 248

EP - 256

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 1-2

ER -