Tubular catabolism of albumin is associated with the release of an inflammatory lipid

Deborah Kees-Folts, Jenny Levis Sadow, George F. Schreiner

Research output: Contribution to journalArticlepeer-review

138 Scopus citations

Abstract

Proteinuria and tubulointerstitial inflammation (TII) correlate with progression to renal failure in human glomerulonephritis. Various forms of experimental nephrotic syndrome are associated with TII. To study the genesis of TII, we utilized the model of albumin overload. Rats received intraperitoneal bovine serum albumin (BSA) for 1 to 14 days, developing heavy proteinuria. A predominantly macrophage interstitial infiltrate was present at days 3, 7 and 14. The urine of the rats contained a factor chemotactic for macrophages which partitioned into the organic phase with ethyl acetate extraction. TLC and HPLC characteristics were those of a novel, non-polar lipid. Supernatant from the culture of proximal tubule (PT) segments after in vivo or in vitro exposure to high concentrations of lipid-replete BSA showed chemotactic activity with similar chromatographic characteristics. PT cultured with delipidated BSA produced little activity. Thus, the generation of this inflammatory factor occurs as a consequence of tubular metabolism of albumin-borne fatty acids and may contribute to the development of proteinuria-associated TII.

Original languageEnglish (US)
Pages (from-to)1697-1709
Number of pages13
JournalKidney International
Volume45
Issue number6
DOIs
StatePublished - Jun 1994

All Science Journal Classification (ASJC) codes

  • Nephrology

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