Tumor cell extravasation mediated by leukocyte adhesion is shear rate dependent on IL-8 signaling

Shile Liang, Meghan Hoskins, Cheng Dong

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

To complete the metastatic journey, cancer cells have to disseminate through the circulation and extravasate to distal organs. However, the extravasation process, by which tumor cells leave a blood vessel and invade the surrounding tissue from the microcirculation, remains poorly understood at the molecular level. In this study, tumor cell adhesion to the endothelium (EC) and subsequent extravasation were investigated under various flow conditions. Results have shown polymorphonuclear neutrophils (PMNs) facilitate melanoma cell adhesion to the EC and subsequent extravasation by a shear-rate dependent mechanism. Melanoma cell-PMN interactions are mediated by the binding between intercellular adhesion molecule-1 (ICAM-1) on melanoma cells and β2 integrins on PMNs. In addition, the fluid convection affects the extent of activation of β2 integrins on PMNs by endogenously secreted interleukin 8 (IL-8) within the tumor microenvironment. Results also indicate that shear rate affects the binding kinetics between PMNs and melanoma cells, which may contribute to the shear-rate dependence of melanoma extravasation in a shear flow when mediated by PMNs.

Original languageEnglish (US)
Pages (from-to)77-91
Number of pages15
JournalMCB Molecular and Cellular Biomechanics
Volume7
Issue number2
StatePublished - 2010

Fingerprint

Interleukin-8
Neutrophils
Leukocytes
Melanoma
Neoplasms
Cell Adhesion
Integrins
Convection
Tumor Microenvironment
Intercellular Adhesion Molecule-1
Microcirculation
Endothelium
Blood Vessels

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Molecular Medicine
  • Molecular Biology
  • Cell Biology

Cite this

@article{c233b606e80b4cb0a29acb5597a17bab,
title = "Tumor cell extravasation mediated by leukocyte adhesion is shear rate dependent on IL-8 signaling",
abstract = "To complete the metastatic journey, cancer cells have to disseminate through the circulation and extravasate to distal organs. However, the extravasation process, by which tumor cells leave a blood vessel and invade the surrounding tissue from the microcirculation, remains poorly understood at the molecular level. In this study, tumor cell adhesion to the endothelium (EC) and subsequent extravasation were investigated under various flow conditions. Results have shown polymorphonuclear neutrophils (PMNs) facilitate melanoma cell adhesion to the EC and subsequent extravasation by a shear-rate dependent mechanism. Melanoma cell-PMN interactions are mediated by the binding between intercellular adhesion molecule-1 (ICAM-1) on melanoma cells and β2 integrins on PMNs. In addition, the fluid convection affects the extent of activation of β2 integrins on PMNs by endogenously secreted interleukin 8 (IL-8) within the tumor microenvironment. Results also indicate that shear rate affects the binding kinetics between PMNs and melanoma cells, which may contribute to the shear-rate dependence of melanoma extravasation in a shear flow when mediated by PMNs.",
author = "Shile Liang and Meghan Hoskins and Cheng Dong",
year = "2010",
language = "English (US)",
volume = "7",
pages = "77--91",
journal = "MCB Molecular and Cellular Biomechanics",
issn = "1556-5297",
publisher = "Tech Science Press",
number = "2",

}

Tumor cell extravasation mediated by leukocyte adhesion is shear rate dependent on IL-8 signaling. / Liang, Shile; Hoskins, Meghan; Dong, Cheng.

In: MCB Molecular and Cellular Biomechanics, Vol. 7, No. 2, 2010, p. 77-91.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Tumor cell extravasation mediated by leukocyte adhesion is shear rate dependent on IL-8 signaling

AU - Liang, Shile

AU - Hoskins, Meghan

AU - Dong, Cheng

PY - 2010

Y1 - 2010

N2 - To complete the metastatic journey, cancer cells have to disseminate through the circulation and extravasate to distal organs. However, the extravasation process, by which tumor cells leave a blood vessel and invade the surrounding tissue from the microcirculation, remains poorly understood at the molecular level. In this study, tumor cell adhesion to the endothelium (EC) and subsequent extravasation were investigated under various flow conditions. Results have shown polymorphonuclear neutrophils (PMNs) facilitate melanoma cell adhesion to the EC and subsequent extravasation by a shear-rate dependent mechanism. Melanoma cell-PMN interactions are mediated by the binding between intercellular adhesion molecule-1 (ICAM-1) on melanoma cells and β2 integrins on PMNs. In addition, the fluid convection affects the extent of activation of β2 integrins on PMNs by endogenously secreted interleukin 8 (IL-8) within the tumor microenvironment. Results also indicate that shear rate affects the binding kinetics between PMNs and melanoma cells, which may contribute to the shear-rate dependence of melanoma extravasation in a shear flow when mediated by PMNs.

AB - To complete the metastatic journey, cancer cells have to disseminate through the circulation and extravasate to distal organs. However, the extravasation process, by which tumor cells leave a blood vessel and invade the surrounding tissue from the microcirculation, remains poorly understood at the molecular level. In this study, tumor cell adhesion to the endothelium (EC) and subsequent extravasation were investigated under various flow conditions. Results have shown polymorphonuclear neutrophils (PMNs) facilitate melanoma cell adhesion to the EC and subsequent extravasation by a shear-rate dependent mechanism. Melanoma cell-PMN interactions are mediated by the binding between intercellular adhesion molecule-1 (ICAM-1) on melanoma cells and β2 integrins on PMNs. In addition, the fluid convection affects the extent of activation of β2 integrins on PMNs by endogenously secreted interleukin 8 (IL-8) within the tumor microenvironment. Results also indicate that shear rate affects the binding kinetics between PMNs and melanoma cells, which may contribute to the shear-rate dependence of melanoma extravasation in a shear flow when mediated by PMNs.

UR - http://www.scopus.com/inward/record.url?scp=77952999313&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77952999313&partnerID=8YFLogxK

M3 - Article

C2 - 20379392

AN - SCOPUS:77952999313

VL - 7

SP - 77

EP - 91

JO - MCB Molecular and Cellular Biomechanics

JF - MCB Molecular and Cellular Biomechanics

SN - 1556-5297

IS - 2

ER -