Tumor suppressor p53 is a regulator of bcl-2 and bax gene expression in vitro and in vivo

Toshiyuki Miyashita, Stanislaw Krajewski, Maryla Krajewska, Hong Gang Wang, H. K. Lin, Dan A. Liebermann, Barbara Hoffman, John C. Reed

Research output: Contribution to journalArticlepeer-review

2105 Scopus citations

Abstract

The p53 tumor suppressor gene product can induce apoptotic cell death through an unknown mechanism. Here we demonstrate that a temperature-sensitive p53 induces temperature-dependent decreases in the expression of the apoptosis-suppressing gene bcl-2 in the murine leukemia cell M1, while simultaneously stimulating increases in the expression of bax, a gene which encodes a dominant-inhibitor of the Bcl-2 protein. Mice deficient in p53 exhibit increases in Bcl-2 and decreases in Bax protein levels in several tissues as determined by immunohistochemical and immunoblot methods. The findings suggest a potential mechanism by which p53 regulates apoptosis, as well as responses to radiation and chemotherapeutic drugs in cancer.

Original languageEnglish (US)
Pages (from-to)1799-1805
Number of pages7
JournalOncogene
Volume9
Issue number6
StatePublished - Jun 1994

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cancer Research

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