Tunneling and Coupled Motion in the Escherichia coli Dihydrofolate Reductase Catalysis

R. Steven Sikorski, Lin Wang, Kelli A. Markham, P. T.Ravi Rajagopalan, Stephen J. Benkovic, Amnon Kohen

Research output: Contribution to journalArticle

161 Citations (Scopus)

Abstract

H-transfer was studied in the complex kinetic cascade of dihydrofolate reductase. Intrinsic kinetic isotope effects, their temperature dependence, and other temperature-dependent parameters indicated H-tunneling, but no 1° to 2° coupled motion. The data also suggested environmentally coupled tunneling and commitment to catalysis on pre-steady-state isotope effects.

Original languageEnglish (US)
Pages (from-to)4778-4779
Number of pages2
JournalJournal of the American Chemical Society
Volume126
Issue number15
DOIs
StatePublished - Apr 21 2004

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Tetrahydrofolate Dehydrogenase
Catalysis
Isotopes
Escherichia coli
Kinetics
Temperature
Thermal effects
Oxidoreductases

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Sikorski, R. S., Wang, L., Markham, K. A., Rajagopalan, P. T. R., Benkovic, S. J., & Kohen, A. (2004). Tunneling and Coupled Motion in the Escherichia coli Dihydrofolate Reductase Catalysis. Journal of the American Chemical Society, 126(15), 4778-4779. https://doi.org/10.1021/ja031683w
Sikorski, R. Steven ; Wang, Lin ; Markham, Kelli A. ; Rajagopalan, P. T.Ravi ; Benkovic, Stephen J. ; Kohen, Amnon. / Tunneling and Coupled Motion in the Escherichia coli Dihydrofolate Reductase Catalysis. In: Journal of the American Chemical Society. 2004 ; Vol. 126, No. 15. pp. 4778-4779.
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Sikorski, RS, Wang, L, Markham, KA, Rajagopalan, PTR, Benkovic, SJ & Kohen, A 2004, 'Tunneling and Coupled Motion in the Escherichia coli Dihydrofolate Reductase Catalysis', Journal of the American Chemical Society, vol. 126, no. 15, pp. 4778-4779. https://doi.org/10.1021/ja031683w

Tunneling and Coupled Motion in the Escherichia coli Dihydrofolate Reductase Catalysis. / Sikorski, R. Steven; Wang, Lin; Markham, Kelli A.; Rajagopalan, P. T.Ravi; Benkovic, Stephen J.; Kohen, Amnon.

In: Journal of the American Chemical Society, Vol. 126, No. 15, 21.04.2004, p. 4778-4779.

Research output: Contribution to journalArticle

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Sikorski RS, Wang L, Markham KA, Rajagopalan PTR, Benkovic SJ, Kohen A. Tunneling and Coupled Motion in the Escherichia coli Dihydrofolate Reductase Catalysis. Journal of the American Chemical Society. 2004 Apr 21;126(15):4778-4779. https://doi.org/10.1021/ja031683w