Two Arabidopsis thaliana dihydrodipicolinate synthases, DHDPS1 and DHDPS2, are unequally redundant

Susan Jones-Held, Luciana Pimenta Ambrozevicius, Michael Campbell, Bradley Drumheller, Emily Harrington, Thomas Leustek

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In Arabidopsis thalinana (L.) Heynh., DHDPS1 and DHDPS2 encode orthologous dihydrodipicolinate synthases (DHDPS), the first enzyme of the lysine (Lys) biosynthesis pathway. A TDNA insertion mutant of dhdps2 was previously reported to be viable and to accumulate free threonine (Thr). Analysis of additional TDNA insertion lines showed that dhdps1 and dhdps2 mutants are both viable and that whereas dhdps2 mutants accumulate Thr, dhdps1 plants do not. Thr-accumulation was complemented by heterologous expression of Escherichia coli DapA, indicating that the phenotype is due to reduced DHDPS activity in dhdps2. DHDPS1 contributes ∼30% towards the total DHDPS activity in leaves of young plants and DHDPS2 contributes 70%; therefore, the threshold of activity resulting in Thr accumulation lies within this narrow range. dhdps1-dhdps2 double mutants could not be isolated, even after exogenous feeding with Lys. Segregation analysis indicated that gametes lacking functional DHDPS genes are defective, as are embryos. Plants carrying only a single DHDPS2 gene do not accumulate Thr, but they show a gametophytic defect that is partially rescued by Lys application. Despite the accumulation of Thr, dhdps2 seedlings are no more sensitive than wild-type plants to growth inhibition by Lys or the Lys precursor diaminopimelate. They also are not rescued by methionine at growth-inhibitory Lys concentrations. Exogenous application of Lys and methionine to dhdps2 mutants did not reduce the accumulation of Thr.

Original languageEnglish (US)
Pages (from-to)1058-1067
Number of pages10
JournalFunctional Plant Biology
Volume39
Issue number12
DOIs
StatePublished - Dec 1 2012

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threonine
lysine
Arabidopsis thaliana
mutants
methionine
embryo (plant)
growth retardation
germ cells
genes
Arabidopsis
biosynthesis
Escherichia coli
phenotype
seedlings
enzymes
leaves

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

Jones-Held, S., Ambrozevicius, L. P., Campbell, M., Drumheller, B., Harrington, E., & Leustek, T. (2012). Two Arabidopsis thaliana dihydrodipicolinate synthases, DHDPS1 and DHDPS2, are unequally redundant. Functional Plant Biology, 39(12), 1058-1067. https://doi.org/10.1071/FP12169
Jones-Held, Susan ; Ambrozevicius, Luciana Pimenta ; Campbell, Michael ; Drumheller, Bradley ; Harrington, Emily ; Leustek, Thomas. / Two Arabidopsis thaliana dihydrodipicolinate synthases, DHDPS1 and DHDPS2, are unequally redundant. In: Functional Plant Biology. 2012 ; Vol. 39, No. 12. pp. 1058-1067.
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abstract = "In Arabidopsis thalinana (L.) Heynh., DHDPS1 and DHDPS2 encode orthologous dihydrodipicolinate synthases (DHDPS), the first enzyme of the lysine (Lys) biosynthesis pathway. A TDNA insertion mutant of dhdps2 was previously reported to be viable and to accumulate free threonine (Thr). Analysis of additional TDNA insertion lines showed that dhdps1 and dhdps2 mutants are both viable and that whereas dhdps2 mutants accumulate Thr, dhdps1 plants do not. Thr-accumulation was complemented by heterologous expression of Escherichia coli DapA, indicating that the phenotype is due to reduced DHDPS activity in dhdps2. DHDPS1 contributes ∼30{\%} towards the total DHDPS activity in leaves of young plants and DHDPS2 contributes 70{\%}; therefore, the threshold of activity resulting in Thr accumulation lies within this narrow range. dhdps1-dhdps2 double mutants could not be isolated, even after exogenous feeding with Lys. Segregation analysis indicated that gametes lacking functional DHDPS genes are defective, as are embryos. Plants carrying only a single DHDPS2 gene do not accumulate Thr, but they show a gametophytic defect that is partially rescued by Lys application. Despite the accumulation of Thr, dhdps2 seedlings are no more sensitive than wild-type plants to growth inhibition by Lys or the Lys precursor diaminopimelate. They also are not rescued by methionine at growth-inhibitory Lys concentrations. Exogenous application of Lys and methionine to dhdps2 mutants did not reduce the accumulation of Thr.",
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Jones-Held, S, Ambrozevicius, LP, Campbell, M, Drumheller, B, Harrington, E & Leustek, T 2012, 'Two Arabidopsis thaliana dihydrodipicolinate synthases, DHDPS1 and DHDPS2, are unequally redundant', Functional Plant Biology, vol. 39, no. 12, pp. 1058-1067. https://doi.org/10.1071/FP12169

Two Arabidopsis thaliana dihydrodipicolinate synthases, DHDPS1 and DHDPS2, are unequally redundant. / Jones-Held, Susan; Ambrozevicius, Luciana Pimenta; Campbell, Michael; Drumheller, Bradley; Harrington, Emily; Leustek, Thomas.

In: Functional Plant Biology, Vol. 39, No. 12, 01.12.2012, p. 1058-1067.

Research output: Contribution to journalArticle

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T1 - Two Arabidopsis thaliana dihydrodipicolinate synthases, DHDPS1 and DHDPS2, are unequally redundant

AU - Jones-Held, Susan

AU - Ambrozevicius, Luciana Pimenta

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