Two-dimensional HYSCORE spectroscopy reveals a histidine imidazole as the axial ligand to Chl3A in the M688HPsaA genetic variant of Photosystem I

Michael J. Gorka, Elijah Gruszecki, Philip Charles, Vidmantas Kalendra, K. V. Lakshmi, John H. Golbeck

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Recent studies on Photosystem I (PS I) have shown that the six core chlorophyll a molecules are highly coupled, allowing for efficient creation and stabilization of the charge-separated state. One area of particular interest is the identity and function of the primary acceptor, A0, as the factors that influence its ultrafast processes and redox properties are not yet fully elucidated. It was recently shown that A0 exists as a dimer of the closely-spaced Chl2/Chl3 molecules wherein the reduced A0[rad]− state has an asymmetric distribution of electron spin density that favors Chl3. Previous experimental work in which this ligand was changed to a hard base (histidine, M688HPsaA) revealed severely impacted electron transfer processes at both the A0 and A1 acceptors; molecular dynamics simulations further suggested two distinct conformations of PS I in which the His residue coordinates and forms a hydrogen bond to the A0 and A1 cofactors, respectively. In this study, we have applied 2D HYSCORE spectroscopy in conjunction with molecular dynamics simulations and density functional theory calculations to the study of the M688HPsaA variant. Analysis of the hyperfine parameters demonstrates that the His imidazole serves as the axial ligand to the central Mg2+ ion in Chl3A in the M688HPsaA variant. Although the change in ligand identity does not alter delocalization of electron density over the Chl2/Chl3 dimer, a small shift in the asymmetry of delocalization, coupled with the electron withdrawing properties of the ligand, most likely accounts for the inhibition of forward electron transfer in the His-ligated conformation.

Original languageEnglish (US)
Article number148424
JournalBiochimica et Biophysica Acta - Bioenergetics
Issue number7
StatePublished - Jul 1 2021

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Cell Biology

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