Two high-resolution crytal sructures of the recombinant N-lobe of human tranferrin reveal a structure change implicated in iron release

Ross T.A. MacGillivray, Stanley A. Moore, Jie Chen, Bryan F. Anderson, Heather Baker, Yaoguang Luo, Maria Bewley, Clyde A. Smith, Michael E.P. Murphy, Yili Wang, Anne B. Mason, Robert C. Woodworth, Gary D. Brayer, Edward N. Baker

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Abstract

The N-lobe of human serum transferrin (hTF/2N) has been expressed in baby hamster kidney cells and crystallized in both orthorhombic (P212121) and tetragonal (P41212) space groups. Both crystal forms diffract to high resolution (1.6 and 1.8 Å, respectively) and have been solved by molecular replacement. Subsequent refinement resulted in final models for the structure of hTF/2N that had crystallographic R-factors of 18.1 and 19.7% for the two crystal forms, respectively; these models represent the highest-resolution transferrin structures determined to date. The hTF/2N polypeptide has a folding pattern similar to those of other transferrins, including the presence of a deep cleft that contains the metal-binding site. In contrast to other transferrins, including the presence of a deep cleft that contains the metal-binding site. In contrast to other transferrins, both crystal forms of hTF/2N display disorder at the iron-binding site; model building suggests hat this disorder consists of alternative conformations of the synergistically bound carbonate anion, the side chain for Arg-124, and several solvent molecules. Subsequent refinement revealed that conformation A has an occupancy of 0.63-0.65 and corresponds to the structure of the iron-binding site found in other transferrins. The alternative conformation B has an occupancy of 0.35-0.37; in this structure, the carbonate has rotated 30°relative to the iron and the side chain for Arg-124 has moved to accommodate the new carbonate position. Several water molecules appear to stabilize the carbonate anion in the two conformations. These structures are consistent with the protonation of the carbonate and resulting partial removal of the anion from the metal; these events would occur prior to cleft opening and metal release.

Original languageEnglish (US)
Pages (from-to)7919-7928
Number of pages10
JournalBiochemistry
Volume37
Issue number22
DOIs
StatePublished - Jun 2 1998

All Science Journal Classification (ASJC) codes

  • Biochemistry

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    MacGillivray, R. T. A., Moore, S. A., Chen, J., Anderson, B. F., Baker, H., Luo, Y., Bewley, M., Smith, C. A., Murphy, M. E. P., Wang, Y., Mason, A. B., Woodworth, R. C., Brayer, G. D., & Baker, E. N. (1998). Two high-resolution crytal sructures of the recombinant N-lobe of human tranferrin reveal a structure change implicated in iron release. Biochemistry, 37(22), 7919-7928. https://doi.org/10.1021/bi980355j