Type II toxin/antitoxin MqsR/MqsA controls type V toxin/antitoxin GhoT/GhoS

Xiaoxue Wang, Dana M. Lord, Seok Hoon Hong, Wolfgang Peti, Michael J. Benedik, Rebecca Page, Thomas K. Wood

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Toxin endoribonucleases of toxin/antitoxin (TA) systems regulate protein production by selectively degrading mRNAs but have never been shown to control other TA systems. Here we demonstrate that toxin MqsR of the MqsR/MqsA system enriches toxin ghoT mRNAin vivo and in vitro, since this transcript lacks the primary MqsR cleavage site 5′-GCU. GhoT is a membrane toxin that causes the ghost cell phenotype, and is part of a type V TA system with antitoxin GhoS that cleaves specifically ghoT mRNA. Introduction of MqsR primary 5′-GCU cleavage sites into ghoT mRNA reduces ghost cell production and cell death likely due to increased degradation of the altered ghoT mRNA by MqsR. GhoT also prevents cell elongation upon the addition of low levels of ampicillin. Therefore, during stress, antitoxin GhoS mRNA is degraded by toxin MqsR allowing ghoT mRNA translation to yield another free toxin that forms ghost cells and increases persistence. Hence, we show that GhoT/GhoS is the first TA system regulated by another TA system.

Original languageEnglish (US)
Pages (from-to)1734-1744
Number of pages11
JournalEnvironmental microbiology
Volume15
Issue number6
DOIs
StatePublished - Jun 1 2013

Fingerprint

antitoxins
Antitoxins
toxin
toxins
Messenger RNA
Endoribonucleases
cleavage
Protein Biosynthesis
Ampicillin
toxin V
endoribonucleases
Cell Death
cells
ampicillin
Phenotype
Membranes
translation (genetics)
phenotype
cell death
cell growth

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Wang, Xiaoxue ; Lord, Dana M. ; Hong, Seok Hoon ; Peti, Wolfgang ; Benedik, Michael J. ; Page, Rebecca ; Wood, Thomas K. / Type II toxin/antitoxin MqsR/MqsA controls type V toxin/antitoxin GhoT/GhoS. In: Environmental microbiology. 2013 ; Vol. 15, No. 6. pp. 1734-1744.
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Wang, X, Lord, DM, Hong, SH, Peti, W, Benedik, MJ, Page, R & Wood, TK 2013, 'Type II toxin/antitoxin MqsR/MqsA controls type V toxin/antitoxin GhoT/GhoS', Environmental microbiology, vol. 15, no. 6, pp. 1734-1744. https://doi.org/10.1111/1462-2920.12063

Type II toxin/antitoxin MqsR/MqsA controls type V toxin/antitoxin GhoT/GhoS. / Wang, Xiaoxue; Lord, Dana M.; Hong, Seok Hoon; Peti, Wolfgang; Benedik, Michael J.; Page, Rebecca; Wood, Thomas K.

In: Environmental microbiology, Vol. 15, No. 6, 01.06.2013, p. 1734-1744.

Research output: Contribution to journalArticle

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