Tyrosine hydroxylase and tryptophan hydroxylase do not form heterotetramers

Susan M. Mockus, George J. Yohrling IV, Kent Vrana

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Tyrosine hydroxylase (TH) and tryptophan hydroxylase (TPH) both contain a C-terminal tetramerization domain composed of a leucine heptad repeat embedded within a 4,3-hydrophobic repeat. Previous mutagenesis experiments and X-ray crystallographic studies have demonstrated that these repeats are required for tetramer assembly of the hydroxylase enzymes via coiled-coil interactions. The specificity of these particular C-terminal intersubunit binding motifs was investigated by determining if TH and TPH can form heterotetramers when coexpressed in bacteria. Bacterial cells were cotransformed with TH and TPH expression plasmids under kanamycin and ampicillin selection, respectively. Immunoprecipitation of induced bacterial supernatants with a TPH monoclonal antibody demonstrated that, unlike the human TH isoforms, TH and TPH do not form heterotetramers. The data suggest that specificity of oligomerization of the aromatic amino acid hydroxylases may be partially determined by polar amino acids interspersed within the coiled-coil. This finding should be influential in the development of eukaryotic expression systems and ultimately in gene therapy approaches.

Original languageEnglish (US)
Pages (from-to)45-51
Number of pages7
JournalJournal of Molecular Neuroscience
Volume10
Issue number1
DOIs
StatePublished - Jan 1 1998

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Tryptophan Hydroxylase
Tyrosine 3-Monooxygenase
Mixed Function Oxygenases
Aromatic Amino Acids
Kanamycin
Ampicillin
Immunoprecipitation
Leucine
Mutagenesis
Genetic Therapy
Protein Isoforms
Plasmids
Monoclonal Antibodies
X-Rays
Bacteria
Amino Acids
Enzymes

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience

Cite this

Mockus, Susan M. ; Yohrling IV, George J. ; Vrana, Kent. / Tyrosine hydroxylase and tryptophan hydroxylase do not form heterotetramers. In: Journal of Molecular Neuroscience. 1998 ; Vol. 10, No. 1. pp. 45-51.
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Tyrosine hydroxylase and tryptophan hydroxylase do not form heterotetramers. / Mockus, Susan M.; Yohrling IV, George J.; Vrana, Kent.

In: Journal of Molecular Neuroscience, Vol. 10, No. 1, 01.01.1998, p. 45-51.

Research output: Contribution to journalArticle

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