UAS(rpg) can function as a heterochromatin boundary element in yeast

Xin Bi, James Broach

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

The HM loci in Saccharomyces cerevisiae constitute region-specific but gene-nonspecific repression domains, as a number of heterologous genes transcribed by RNA polymerase II or III are silenced when placed at these loci. The promoters of the Ashbya gossypii TEF gene and the S. cerevisiae TEF1 and TEF2 genes, however, are resistant to transcriptional silencing by the HM silencers in yeast. Moreover, when interposed between the HML α genes and the E silencer, certain segments of these promoters block the repression effect of the silencer on the α genes. All of these fragments contain UAS(rpg) (upstream activation sequence of ribosome protein genes) composed of multiple binding sites for Rap1. In fact, a 149-bp segment consisting essentially of only three tandem Rap1-binding sites from the UAS(rpg) of yeast TEF2 exhibits silencer-blocking activity. This element also exhibits insulating activity and orientation dependence characteristic of known chromatin boundary elements. Finally, the element blocks the physical spread of heterochromatin initiated at a silencer. This segment provides the first example of chromatin domain boundary or insulator elements in yeast.

Original languageEnglish (US)
Pages (from-to)1089-1101
Number of pages13
JournalGenes and Development
Volume13
Issue number9
DOIs
StatePublished - May 1 1999

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Insulator Elements
Heterochromatin
Ribosomes
Yeasts
Genes
Proteins
Chromatin
Saccharomyces cerevisiae
Binding Sites
RNA Polymerase III
Fungal Proteins
RNA Polymerase II

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

Cite this

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abstract = "The HM loci in Saccharomyces cerevisiae constitute region-specific but gene-nonspecific repression domains, as a number of heterologous genes transcribed by RNA polymerase II or III are silenced when placed at these loci. The promoters of the Ashbya gossypii TEF gene and the S. cerevisiae TEF1 and TEF2 genes, however, are resistant to transcriptional silencing by the HM silencers in yeast. Moreover, when interposed between the HML α genes and the E silencer, certain segments of these promoters block the repression effect of the silencer on the α genes. All of these fragments contain UAS(rpg) (upstream activation sequence of ribosome protein genes) composed of multiple binding sites for Rap1. In fact, a 149-bp segment consisting essentially of only three tandem Rap1-binding sites from the UAS(rpg) of yeast TEF2 exhibits silencer-blocking activity. This element also exhibits insulating activity and orientation dependence characteristic of known chromatin boundary elements. Finally, the element blocks the physical spread of heterochromatin initiated at a silencer. This segment provides the first example of chromatin domain boundary or insulator elements in yeast.",
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UAS(rpg) can function as a heterochromatin boundary element in yeast. / Bi, Xin; Broach, James.

In: Genes and Development, Vol. 13, No. 9, 01.05.1999, p. 1089-1101.

Research output: Contribution to journalArticle

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