Ubiquitin‐Directed Antibodies Inhibit Neuronal Transporters in Rat Brain Synaptosomes

Edwin M. Meyer, Christopher M. West, Bruce R. Stevens, Vincent Chau, Minh‐thanh ‐t Nguyen, Jennifer H. Judkins

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Affinity‐purified antibodies specific for ubiquitin were found to inhibit the sodium‐dependent uptake of [3H]choline, r‐[3H]aminobutyric acid ([3H]GABA), [3H]‐glutamate, [3H]norepinephrine, [3H]aspartate, and [3H]scrotonin in rat cerebral cortical synaptosomes at a low concentration (10 μg/ml). These antibodies (termed anti‐Uh) had no effect on the sodium‐independent uptake of these substances or their calcium‐dependent efflux. Synaptosomal [3H]deoxyglucose uptake was not affected in normal Krebs Ringer buffer containing 10 mM glucose, but was inhibited in glucose‐free medium. Other nonneuronal sodium‐dependent transport processes were found to be unaffected by 10 μg/ml anti‐Ub, suggesting that anti‐Ub does not bind indiscriminantly to sodium‐binding sites on sodium‐dependent organic solute transporters. Finally, anti‐Ub inhibited sodium‐dependent [3H]GABA and [3H]glutamate uptake in plasma membrane ghosts, devoid of membrane potential, which were derived from rat cerebral cortical synaptosomes. These results suggest that neuronal transporters or sites proximal to them may be ubiquitinylated on the plasma membrane surface.

Original languageEnglish (US)
Pages (from-to)1815-1819
Number of pages5
JournalJournal of neurochemistry
Volume49
Issue number6
DOIs
StatePublished - Dec 1987

Fingerprint

Synaptosomes
Cell membranes
gamma-Aminobutyric Acid
Rats
Glutamic Acid
Brain
Cell Membrane
Aminobutyrates
Antibodies
Deoxyglucose
Ubiquitin
Choline
Aspartic Acid
Membrane Potentials
Norepinephrine
Buffers
Membranes
Glucose

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Meyer, Edwin M. ; M. West, Christopher ; Stevens, Bruce R. ; Chau, Vincent ; Nguyen, Minh‐thanh ‐t ; Judkins, Jennifer H. / Ubiquitin‐Directed Antibodies Inhibit Neuronal Transporters in Rat Brain Synaptosomes. In: Journal of neurochemistry. 1987 ; Vol. 49, No. 6. pp. 1815-1819.
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Ubiquitin‐Directed Antibodies Inhibit Neuronal Transporters in Rat Brain Synaptosomes. / Meyer, Edwin M.; M. West, Christopher; Stevens, Bruce R.; Chau, Vincent; Nguyen, Minh‐thanh ‐t; Judkins, Jennifer H.

In: Journal of neurochemistry, Vol. 49, No. 6, 12.1987, p. 1815-1819.

Research output: Contribution to journalArticle

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