Unambiguous Identification of β-Tubulin as the Direct Cellular Target Responsible for the Cytotoxicity of Chalcone by Photoaffinity Labeling

Bo Zhou, Xingxin Yu, Chunlin Zhuang, Peter Villalta, Yong Lin, Junxuan Lu, Chengguo Xing

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Chalcone is a simple and potentially privileged structure in medicinal chemistry with a diverse repertoire of biological activities, among which cytotoxicity is of particular interest. The sharp structure–activity relationship (SAR) for chalcone's cytotoxicity suggests structure-specific target interactions. Despite the numerous putative targets proposed, evidence for direct target interactions in cells is unavailable. In this study, guided by the sharp cytotoxic SAR, we developed a cytotoxic chalcone-based photoaffinity labeling (PAL) probe, (E)-3-(3-azidophenyl)-1-[3,5-dimethoxy-4-(prop-2-yn-1-yloxy)phenyl]-2-methylprop-2-en-1-one (C95; IC50: 0.38±0.01 μm), along with two structurally similar non-cytotoxic probes. These probes were used to search for the direct cellular target responsible for chalcone's cytotoxicity through intact cell-based PAL experiments, in which β-tubulin was identified to specifically interact with the cytotoxic probe (i.e., C95) but not the non-cytotoxic probes. A set of phenotypical and biochemical assays further reinforced β-tubulin as the cytotoxic target of chalcones. Peptide mass quantitation by mass spectrometric analysis revealed one peptide potentially labeled by C95, providing information on chalcone's binding site on β-tubulin.

Original languageEnglish (US)
Pages (from-to)1436-1445
Number of pages10
JournalChemMedChem
Volume11
Issue number13
DOIs
StatePublished - Jan 1 2016

Fingerprint

Chalcone
Tubulin
Cytotoxicity
Labeling
Chalcones
Peptides
Pharmaceutical Chemistry
Cell Communication
Inhibitory Concentration 50
Binding Sites
Bioactivity
Assays
Experiments

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Pharmacology
  • Drug Discovery
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Organic Chemistry

Cite this

Zhou, Bo ; Yu, Xingxin ; Zhuang, Chunlin ; Villalta, Peter ; Lin, Yong ; Lu, Junxuan ; Xing, Chengguo. / Unambiguous Identification of β-Tubulin as the Direct Cellular Target Responsible for the Cytotoxicity of Chalcone by Photoaffinity Labeling. In: ChemMedChem. 2016 ; Vol. 11, No. 13. pp. 1436-1445.
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Unambiguous Identification of β-Tubulin as the Direct Cellular Target Responsible for the Cytotoxicity of Chalcone by Photoaffinity Labeling. / Zhou, Bo; Yu, Xingxin; Zhuang, Chunlin; Villalta, Peter; Lin, Yong; Lu, Junxuan; Xing, Chengguo.

In: ChemMedChem, Vol. 11, No. 13, 01.01.2016, p. 1436-1445.

Research output: Contribution to journalArticle

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