Uncharacterized Escherichia coli proteins YdjA and YhjY are related to biohydrogen production

Mohd Zulkhairi Mohd Yusoff, Toshinari Maeda, Viviana Sanchez-Torres, Hiroaki I. Ogawa, Yoshihito Shirai, Mohd Ali Hassan, Thomas K. Wood

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Biohydrogen has gained importance as an alternative energy source, and advances in molecular biology and biotechnology have raised the quality and efficiency of biohydrogen production from various microorganisms and substrates. Here, Escherichia coli proteins YdjA and YhjY have been identified as essential in biohydrogen production from glucose. The mutations ydjA and yhjY reduced biohydrogen productivity compared to the parent strain from 40 to 4 and 29 μmol/mg protein, respectively. Through transcription analysis, it was determined that YdjA and YhjY are positive effectors of the FHL complex since their inactivation repressed fhlA. In addition, the FHL expression of the repressor gene, hycA, increased for the ydjA mutant, so YdjA reduces transcription of the HycA repressor. Hence, two new proteins have been identified that are important for biohydrogen production.

Original languageEnglish (US)
Pages (from-to)17778-17787
Number of pages10
JournalInternational Journal of Hydrogen Energy
Volume37
Issue number23
DOIs
StatePublished - Dec 2012

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

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