Unencapsidated double-stranded RNA associated with membrane vesicles in isolates of Alternaria solani

I. Zabalgogeazcoa, D. Petrunak, Barbara Jane Christ, F. E. Gildow

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Double-stranded RNA (dsRNA) elements of 8·3 and 5·5 kbp were detected in three of five geographically distinct isolates of Alternaria solani. Variation with respect to the number of dsRNA segments was detected among isolates of the fungus. No virus-like particles were purified from the mycelium, or observed in ultra-thin sections of hyphae. Utilizing differential centrifugation or density gradient centrifugation for subcellular fractionation, dsRNA was found associated specifically with fractions containing 80-100 nm spherical membrane vesicles. Similar vesicles were observed in cytoplasm of hyphae by electron microscopy. Treatment of dsRNA-containing fractions with ribonuclease A did not degrade the dsRNA, suggesting that it is located inside the vesicles. There was no correlation between the radial growth of the isolates in laboratory media and the presence or absence or dsRNA.

Original languageEnglish (US)
Pages (from-to)604-608
Number of pages5
JournalMycological Research
Volume101
Issue number5
DOIs
StatePublished - Jan 1 1997

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Plant Science

Fingerprint Dive into the research topics of 'Unencapsidated double-stranded RNA associated with membrane vesicles in isolates of Alternaria solani'. Together they form a unique fingerprint.

Cite this