Unique inducible filamentous motility identified in pathogenic Bacillus cereus group species

Martha M. Liu, Shannon Coleman, Lauren Wilkinson, Maren L. Smith, Thomas Hoang, Naomi Niyah, Manjari Mukherjee, Steven Huynh, Craig T. Parker, Jasna Kovac, Robert E.W. Hancock, Erin C. Gaynor

Research output: Contribution to journalArticlepeer-review

Abstract

Active migration across semi-solid surfaces is important for bacterial success by facilitating colonization of unoccupied niches and is often associated with altered virulence and antibiotic resistance profiles. We isolated an atmospheric contaminant, subsequently identified as a new strain of Bacillus mobilis, which showed a unique, robust, rapid, and inducible filamentous surface motility. This flagella-independent migration was characterized by formation of elongated cells at the expanding edge and was induced when cells were inoculated onto lawns of metabolically inactive Campylobacter jejuni cells, autoclaved bacterial biomass, adsorbed milk, and adsorbed blood atop hard agar plates. Phosphatidylcholine (PC), bacterial membrane components, and sterile human fecal extracts were also sufficient to induce filamentous expansion. Screening of eight other Bacillus spp. showed that filamentous motility was conserved amongst B. cereus group species to varying degrees. RNA-Seq of elongated expanding cells collected from adsorbed milk and PC lawns versus control rod-shaped cells revealed dysregulation of genes involved in metabolism and membrane transport, sporulation, quorum sensing, antibiotic synthesis, and virulence (e.g., hblA/B/C/D and plcR). These findings characterize the robustness and ecological significance of filamentous surface motility in B. cereus group species and lay the foundation for understanding the biological role it may play during environment and host colonization.

Original languageEnglish (US)
Pages (from-to)2997-3010
Number of pages14
JournalISME Journal
Volume14
Issue number12
DOIs
StatePublished - Dec 1 2020

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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