Unnatural translation initiation

John W. Tomsho, Stephen Benkovic

Research output: Contribution to journalArticle

Abstract

Protein translation in nature always begins with an initiator transfer RNA (tRNA) carrying the amino acid methionine. This was circumvented in vitro with a reconstituted translation system utilizing initiator tRNA synthetically mischarged with the other natural amino acids. In addition, it was determined that this system could accommodate these non-methionine amino acids containing various N-α-acyl groups, many of which are useful for post-translational modification such as peptide cyclization.

Original languageEnglish (US)
Pages (from-to)87-88
Number of pages2
JournalACS chemical biology
Volume3
Issue number2
DOIs
StatePublished - Feb 1 2008

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RNA, Transfer, Met
Amino Acids
Cyclization
Protein Biosynthesis
Post Translational Protein Processing
Methionine
Peptides
Proteins
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine

Cite this

Tomsho, John W. ; Benkovic, Stephen. / Unnatural translation initiation. In: ACS chemical biology. 2008 ; Vol. 3, No. 2. pp. 87-88.
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Tomsho, JW & Benkovic, S 2008, 'Unnatural translation initiation', ACS chemical biology, vol. 3, no. 2, pp. 87-88. https://doi.org/10.1021/cb8000233

Unnatural translation initiation. / Tomsho, John W.; Benkovic, Stephen.

In: ACS chemical biology, Vol. 3, No. 2, 01.02.2008, p. 87-88.

Research output: Contribution to journalArticle

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