Unrip is a component of SMN complexes active in snRNP assembly

Claudia Carissimi, Jennifer Baccon, Marco Straccia, Pieranna Chiarella, Alessio Maiolica, Alan Sawyer, Juri Rappsilber, Livio Pellizzoni

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

A macromolecular complex containing survival of motor neurons (SMN), the spinal muscular atrophy protein, and Gemin2-7 interacts with Sm proteins and snRNAs to carry out the assembly of these components into spliceosomal small nuclear ribonucleoproteins (snRNPs). Here we report the characterization of unr-interacting protein (unrip), a GH-WD protein of unknown function, as a component of the SMN complex that interacts directly with Gemin6 and Gemin7. Unrip also binds a subset of Sm proteins, and unrip-containing SMN complexes are necessary and sufficient to mediate the assembly of spliceosomal snRNPs. These results demonstrate that unrip functions in the pathway of snRNP biogenesis and is a marker of cellular SMN complexes active in snRNP assembly.

Original languageEnglish (US)
Pages (from-to)2348-2354
Number of pages7
JournalFEBS Letters
Volume579
Issue number11
DOIs
StatePublished - Apr 25 2005

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SMN Complex Proteins
Small Nuclear Ribonucleoproteins
Proteins
Macromolecular Substances
Spinal Muscular Atrophy
Motor Neurons
Neurons

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Carissimi, C., Baccon, J., Straccia, M., Chiarella, P., Maiolica, A., Sawyer, A., ... Pellizzoni, L. (2005). Unrip is a component of SMN complexes active in snRNP assembly. FEBS Letters, 579(11), 2348-2354. https://doi.org/10.1016/j.febslet.2005.03.034
Carissimi, Claudia ; Baccon, Jennifer ; Straccia, Marco ; Chiarella, Pieranna ; Maiolica, Alessio ; Sawyer, Alan ; Rappsilber, Juri ; Pellizzoni, Livio. / Unrip is a component of SMN complexes active in snRNP assembly. In: FEBS Letters. 2005 ; Vol. 579, No. 11. pp. 2348-2354.
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Carissimi, C, Baccon, J, Straccia, M, Chiarella, P, Maiolica, A, Sawyer, A, Rappsilber, J & Pellizzoni, L 2005, 'Unrip is a component of SMN complexes active in snRNP assembly', FEBS Letters, vol. 579, no. 11, pp. 2348-2354. https://doi.org/10.1016/j.febslet.2005.03.034

Unrip is a component of SMN complexes active in snRNP assembly. / Carissimi, Claudia; Baccon, Jennifer; Straccia, Marco; Chiarella, Pieranna; Maiolica, Alessio; Sawyer, Alan; Rappsilber, Juri; Pellizzoni, Livio.

In: FEBS Letters, Vol. 579, No. 11, 25.04.2005, p. 2348-2354.

Research output: Contribution to journalArticle

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AU - Carissimi, Claudia

AU - Baccon, Jennifer

AU - Straccia, Marco

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AU - Sawyer, Alan

AU - Rappsilber, Juri

AU - Pellizzoni, Livio

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Carissimi C, Baccon J, Straccia M, Chiarella P, Maiolica A, Sawyer A et al. Unrip is a component of SMN complexes active in snRNP assembly. FEBS Letters. 2005 Apr 25;579(11):2348-2354. https://doi.org/10.1016/j.febslet.2005.03.034