Upregulation of pigment epithelium-derived factor after laser photocoagulation

Nahoko Ogata, Joyce Tombran-Tink, Nobuo Jo, David Mrazek, Miyo Matsumura

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

PURPOSE: To determine the changes in the expression of pigment epithelium-derived factor in cultured human retinal pigment epithelial cells and rat retinas after laser photocoagulation. METHODS: Experimental study of laser photocoagulation on human retinal pigment epithelial cells in culture and on adult rats. Reverse transcription-polymerase chain reaction and semiquantitative polymerase chain reaction analysis were used. RESULTS: After photocoagulation, the mRNA expression of pigment epithelium-derived factor was upregulated in human retinal pigment epithelial cells at 6 hours and then gradually decreased. Compared with controls, significantly higher levels of pigment epithelium-derived factor were observed in rat retinas from 6 to 24 hours after laser photocoagulation (P < .005), and they were still higher than before photocoagulation at 2 weeks. CONCLUSION: An upregulation of pigment epithelium-derived factor in retinal pigment epithelial cells and in the retina after photocoagulation suggests that pigment epithelium-derived factor plays a role in inhibiting neovascularization by its antiangiogenic activity.

Original languageEnglish (US)
Pages (from-to)427-429
Number of pages3
JournalAmerican Journal of Ophthalmology
Volume132
Issue number3
DOIs
StatePublished - Aug 31 2001

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Light Coagulation
Lasers
Retinal Pigments
Up-Regulation
Epithelial Cells
Retina
Polymerase Chain Reaction
Reverse Transcription
pigment epithelium-derived factor
Cell Culture Techniques
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Ophthalmology

Cite this

Ogata, Nahoko ; Tombran-Tink, Joyce ; Jo, Nobuo ; Mrazek, David ; Matsumura, Miyo. / Upregulation of pigment epithelium-derived factor after laser photocoagulation. In: American Journal of Ophthalmology. 2001 ; Vol. 132, No. 3. pp. 427-429.
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Upregulation of pigment epithelium-derived factor after laser photocoagulation. / Ogata, Nahoko; Tombran-Tink, Joyce; Jo, Nobuo; Mrazek, David; Matsumura, Miyo.

In: American Journal of Ophthalmology, Vol. 132, No. 3, 31.08.2001, p. 427-429.

Research output: Contribution to journalArticle

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N2 - PURPOSE: To determine the changes in the expression of pigment epithelium-derived factor in cultured human retinal pigment epithelial cells and rat retinas after laser photocoagulation. METHODS: Experimental study of laser photocoagulation on human retinal pigment epithelial cells in culture and on adult rats. Reverse transcription-polymerase chain reaction and semiquantitative polymerase chain reaction analysis were used. RESULTS: After photocoagulation, the mRNA expression of pigment epithelium-derived factor was upregulated in human retinal pigment epithelial cells at 6 hours and then gradually decreased. Compared with controls, significantly higher levels of pigment epithelium-derived factor were observed in rat retinas from 6 to 24 hours after laser photocoagulation (P < .005), and they were still higher than before photocoagulation at 2 weeks. CONCLUSION: An upregulation of pigment epithelium-derived factor in retinal pigment epithelial cells and in the retina after photocoagulation suggests that pigment epithelium-derived factor plays a role in inhibiting neovascularization by its antiangiogenic activity.

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