Upregulation of pigment epithelium-derived factor after laser photocoagulation

Nahoko Ogata, Joyce Tombran-Tink, Nobuo Jo, David Mrazek, Miyo Matsumura

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Abstract

PURPOSE: To determine the changes in the expression of pigment epithelium-derived factor in cultured human retinal pigment epithelial cells and rat retinas after laser photocoagulation. METHODS: Experimental study of laser photocoagulation on human retinal pigment epithelial cells in culture and on adult rats. Reverse transcription-polymerase chain reaction and semiquantitative polymerase chain reaction analysis were used. RESULTS: After photocoagulation, the mRNA expression of pigment epithelium-derived factor was upregulated in human retinal pigment epithelial cells at 6 hours and then gradually decreased. Compared with controls, significantly higher levels of pigment epithelium-derived factor were observed in rat retinas from 6 to 24 hours after laser photocoagulation (P < .005), and they were still higher than before photocoagulation at 2 weeks. CONCLUSION: An upregulation of pigment epithelium-derived factor in retinal pigment epithelial cells and in the retina after photocoagulation suggests that pigment epithelium-derived factor plays a role in inhibiting neovascularization by its antiangiogenic activity.

Original languageEnglish (US)
Pages (from-to)427-429
Number of pages3
JournalAmerican Journal of Ophthalmology
Volume132
Issue number3
DOIs
StatePublished - Aug 31 2001

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All Science Journal Classification (ASJC) codes

  • Ophthalmology

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