Urinary retinol excretion in marginally vitamin a deficient rats during inflammation and following supplementation with oil or water-miscible vitamin a

F. J. Rosales, N. Q. Li, A. C. Ross

Research output: Contribution to journalArticlepeer-review

Abstract

To assess whether urinary retinol excretion is due to inflammation or to other causes, vitamin A-marginal and -sufficient rats (4/ group) were treated i.p. with 50 μg of lipopolysaccharide (LPS) from P. aeruginosa/ 100 g of b.w. In a second experiment, 16 marginal rats were treated with LPS or saline, followed six hours later by an oral dose of 2 mg of retinol as oilsoluble (OILVA) or water-miscible (AQUVA) retinyl palmitate (four groups: OILVA alone, AQUVA alone, LPS+OILVA and LPS+AQUVA). Total plasma retinol was determined by HPLC before and 24 h post-treatment, and urinary retinol after 24 h collection. Inflammation with LPS caused hyporetinemia (∼40% reduction in plasma retinol) in both marginal and sufficient rats. However, in none of marginal LPS treated rats was retinol detected in urine (< 4.0 ng/dl), while among sufficient, only one rat excreted measurable retinol. Vitamin A supplementation increased total plasma retinol (∼2 times pre-treatment levels) in each of the four groups. However, urinary retinol was excreted by 50% of rats receiving AQUVA alone (range, 80 to 90 ng/dl) and 100% of those treated with LPS+AQUVA (range, 30 to 320 ng/dl). These results indicate that urinary retinol excretion is not necessarily associated with inflammation, and suggest that AQUVA differs metabolically from OILVA.

Original languageEnglish (US)
Pages (from-to)A466
JournalFASEB Journal
Volume10
Issue number3
StatePublished - Dec 1 1996

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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