Urinary retinol excretion in marginally vitamin a deficient rats during inflammation and following supplementation with oil or water-miscible vitamin a

F. J. Rosales, N. Q. Li, A. C. Ross

Research output: Contribution to journalArticle

Abstract

To assess whether urinary retinol excretion is due to inflammation or to other causes, vitamin A-marginal and -sufficient rats (4/ group) were treated i.p. with 50 μg of lipopolysaccharide (LPS) from P. aeruginosa/ 100 g of b.w. In a second experiment, 16 marginal rats were treated with LPS or saline, followed six hours later by an oral dose of 2 mg of retinol as oilsoluble (OILVA) or water-miscible (AQUVA) retinyl palmitate (four groups: OILVA alone, AQUVA alone, LPS+OILVA and LPS+AQUVA). Total plasma retinol was determined by HPLC before and 24 h post-treatment, and urinary retinol after 24 h collection. Inflammation with LPS caused hyporetinemia (∼40% reduction in plasma retinol) in both marginal and sufficient rats. However, in none of marginal LPS treated rats was retinol detected in urine (< 4.0 ng/dl), while among sufficient, only one rat excreted measurable retinol. Vitamin A supplementation increased total plasma retinol (∼2 times pre-treatment levels) in each of the four groups. However, urinary retinol was excreted by 50% of rats receiving AQUVA alone (range, 80 to 90 ng/dl) and 100% of those treated with LPS+AQUVA (range, 30 to 320 ng/dl). These results indicate that urinary retinol excretion is not necessarily associated with inflammation, and suggest that AQUVA differs metabolically from OILVA.

Original languageEnglish (US)
Pages (from-to)A466
JournalFASEB Journal
Volume10
Issue number3
StatePublished - Dec 1 1996

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Vitamin A
Vitamins
Rats
Oils
Inflammation
Water
Lipopolysaccharides
Plasmas
High Pressure Liquid Chromatography
Urine

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

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title = "Urinary retinol excretion in marginally vitamin a deficient rats during inflammation and following supplementation with oil or water-miscible vitamin a",
abstract = "To assess whether urinary retinol excretion is due to inflammation or to other causes, vitamin A-marginal and -sufficient rats (4/ group) were treated i.p. with 50 μg of lipopolysaccharide (LPS) from P. aeruginosa/ 100 g of b.w. In a second experiment, 16 marginal rats were treated with LPS or saline, followed six hours later by an oral dose of 2 mg of retinol as oilsoluble (OILVA) or water-miscible (AQUVA) retinyl palmitate (four groups: OILVA alone, AQUVA alone, LPS+OILVA and LPS+AQUVA). Total plasma retinol was determined by HPLC before and 24 h post-treatment, and urinary retinol after 24 h collection. Inflammation with LPS caused hyporetinemia (∼40{\%} reduction in plasma retinol) in both marginal and sufficient rats. However, in none of marginal LPS treated rats was retinol detected in urine (< 4.0 ng/dl), while among sufficient, only one rat excreted measurable retinol. Vitamin A supplementation increased total plasma retinol (∼2 times pre-treatment levels) in each of the four groups. However, urinary retinol was excreted by 50{\%} of rats receiving AQUVA alone (range, 80 to 90 ng/dl) and 100{\%} of those treated with LPS+AQUVA (range, 30 to 320 ng/dl). These results indicate that urinary retinol excretion is not necessarily associated with inflammation, and suggest that AQUVA differs metabolically from OILVA.",
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Urinary retinol excretion in marginally vitamin a deficient rats during inflammation and following supplementation with oil or water-miscible vitamin a. / Rosales, F. J.; Li, N. Q.; Ross, A. C.

In: FASEB Journal, Vol. 10, No. 3, 01.12.1996, p. A466.

Research output: Contribution to journalArticle

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