Use of a newly developed rapid microbial ATP bioluminescence assay to detect microbial contamination on poultry carcasses

G. R. Siragusa, W. J. Dorsa, C. N. Cutter, L. J. Perino, M. Koohmaraie

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

A newly developed rapid microbial ATP bioluminescence test (R-mATP) was shown to be an adequate means to assay the microbial load of poultry carcasses. This assay utilizes differential extraction and filtration to separate somatic from microbial ATP in a very rapid timeframe. The assay requires approximately 5 min to complete; approximately 3.5 min to sample and 90 s analytical time. Correlation coefficient (r) between aerobic colony counts and R-mATP test results (n=329) was 0.82. Post-test probabilities to correctly classify carcasses with different levels of microbial contamination were as high as 98% for samples of ≥ 3.5 log aerobic CPU per ml. Given the rapidity of this assay, the R-mATP holds potential for monitoring the microbial load of carcasses at poultry-processing critical control points. Other potential applications of this new version of the microbial ATP bioluminescence test are discussed.

Original languageEnglish (US)
Pages (from-to)297-301
Number of pages5
JournalJournal of Bioluminescence and Chemiluminescence
Volume11
Issue number6
DOIs
StatePublished - 1996

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Chemistry (miscellaneous)

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