Abstract
A newly developed rapid microbial ATP bioluminescence test (R-mATP) was shown to be an adequate means to assay the microbial load of poultry carcasses. This assay utilizes differential extraction and filtration to separate somatic from microbial ATP in a very rapid timeframe. The assay requires approximately 5 min to complete; approximately 3.5 min to sample and 90 s analytical time. Correlation coefficient (r) between aerobic colony counts and R-mATP test results (n=329) was 0.82. Post-test probabilities to correctly classify carcasses with different levels of microbial contamination were as high as 98% for samples of ≥ 3.5 log aerobic CPU per ml. Given the rapidity of this assay, the R-mATP holds potential for monitoring the microbial load of carcasses at poultry-processing critical control points. Other potential applications of this new version of the microbial ATP bioluminescence test are discussed.
Original language | English (US) |
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Pages (from-to) | 297-301 |
Number of pages | 5 |
Journal | Journal of Bioluminescence and Chemiluminescence |
Volume | 11 |
Issue number | 6 |
DOIs | |
State | Published - 1996 |
All Science Journal Classification (ASJC) codes
- Biophysics
- Chemistry (miscellaneous)