Abstract
A new microbial ATP bioluminescence assay was shown to be an accurate and rapid mefJiod to determine the levels of generic bacterial contamination on beef (n = 400) and pork (n = 320) carcasses sampled in commercial processing plants. Based on in vitro fecal dilution studies, the rapid microbial ATP (R-mATP) assay is as accurate as the standard plate count method for estimating bacteria in bovine or porcine fecal samples. The correlations (r) between the R-mATP assay and the standard aerobic plate count for beef and pork carcasses sampled in commercial processing were 0.91 and 0.93, respectively. A segmented-model statistical approach to determine the lower limits of assay sensitivity was developed. By using this model to analyze the in-plant data, the R-mATP test responded in a linear fashion to levels of microbial contamination of > log10 2.0 aerobic CFU/cm2 on beef carcasses and of > log10 3.2 aerobic CFU/cm2 for pork carcasses. The R-mATP assay requires approximately 5 min to complete, including sampling. Given the rapidity and accuracy of the assay, processors interested in monitoring critical control points in the slaughter process could potentially use the R-mATP assay to monitor microbiological prevention and intervention procedures for minimizing carcass contamination.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 770-775 |
| Number of pages | 6 |
| Journal | Journal of Food Protection |
| Volume | 58 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jan 1 1995 |
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All Science Journal Classification (ASJC) codes
- Food Science
- Microbiology
Cite this
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Use of a rapid microbial ATP bioluminescence assay to detect contamination on beef and pork carcasses. / Siragusa, Gregory R.; Cutter, Catherine Nettles; Dorsa, Warren J.; Koohmaraie, Mohammad.
In: Journal of Food Protection, Vol. 58, No. 7, 01.01.1995, p. 770-775.Research output: Contribution to journal › Article
TY - JOUR
T1 - Use of a rapid microbial ATP bioluminescence assay to detect contamination on beef and pork carcasses
AU - Siragusa, Gregory R.
AU - Cutter, Catherine Nettles
AU - Dorsa, Warren J.
AU - Koohmaraie, Mohammad
PY - 1995/1/1
Y1 - 1995/1/1
N2 - A new microbial ATP bioluminescence assay was shown to be an accurate and rapid mefJiod to determine the levels of generic bacterial contamination on beef (n = 400) and pork (n = 320) carcasses sampled in commercial processing plants. Based on in vitro fecal dilution studies, the rapid microbial ATP (R-mATP) assay is as accurate as the standard plate count method for estimating bacteria in bovine or porcine fecal samples. The correlations (r) between the R-mATP assay and the standard aerobic plate count for beef and pork carcasses sampled in commercial processing were 0.91 and 0.93, respectively. A segmented-model statistical approach to determine the lower limits of assay sensitivity was developed. By using this model to analyze the in-plant data, the R-mATP test responded in a linear fashion to levels of microbial contamination of > log10 2.0 aerobic CFU/cm2 on beef carcasses and of > log10 3.2 aerobic CFU/cm2 for pork carcasses. The R-mATP assay requires approximately 5 min to complete, including sampling. Given the rapidity and accuracy of the assay, processors interested in monitoring critical control points in the slaughter process could potentially use the R-mATP assay to monitor microbiological prevention and intervention procedures for minimizing carcass contamination.
AB - A new microbial ATP bioluminescence assay was shown to be an accurate and rapid mefJiod to determine the levels of generic bacterial contamination on beef (n = 400) and pork (n = 320) carcasses sampled in commercial processing plants. Based on in vitro fecal dilution studies, the rapid microbial ATP (R-mATP) assay is as accurate as the standard plate count method for estimating bacteria in bovine or porcine fecal samples. The correlations (r) between the R-mATP assay and the standard aerobic plate count for beef and pork carcasses sampled in commercial processing were 0.91 and 0.93, respectively. A segmented-model statistical approach to determine the lower limits of assay sensitivity was developed. By using this model to analyze the in-plant data, the R-mATP test responded in a linear fashion to levels of microbial contamination of > log10 2.0 aerobic CFU/cm2 on beef carcasses and of > log10 3.2 aerobic CFU/cm2 for pork carcasses. The R-mATP assay requires approximately 5 min to complete, including sampling. Given the rapidity and accuracy of the assay, processors interested in monitoring critical control points in the slaughter process could potentially use the R-mATP assay to monitor microbiological prevention and intervention procedures for minimizing carcass contamination.
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UR - http://www.scopus.com/inward/citedby.url?scp=0028842067&partnerID=8YFLogxK
U2 - 10.4315/0362-028X-58.7.770
DO - 10.4315/0362-028X-58.7.770
M3 - Article
VL - 58
SP - 770
EP - 775
JO - Journal of Food Protection
JF - Journal of Food Protection
SN - 0362-028X
IS - 7
ER -