Use of a rapid microbial ATP bioluminescence assay to detect contamination on beef and pork carcasses

Gregory R. Siragusa, Catherine Nettles Cutter, Warren J. Dorsa, Mohammad Koohmaraie

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

A new microbial ATP bioluminescence assay was shown to be an accurate and rapid mefJiod to determine the levels of generic bacterial contamination on beef (n = 400) and pork (n = 320) carcasses sampled in commercial processing plants. Based on in vitro fecal dilution studies, the rapid microbial ATP (R-mATP) assay is as accurate as the standard plate count method for estimating bacteria in bovine or porcine fecal samples. The correlations (r) between the R-mATP assay and the standard aerobic plate count for beef and pork carcasses sampled in commercial processing were 0.91 and 0.93, respectively. A segmented-model statistical approach to determine the lower limits of assay sensitivity was developed. By using this model to analyze the in-plant data, the R-mATP test responded in a linear fashion to levels of microbial contamination of > log10 2.0 aerobic CFU/cm2 on beef carcasses and of > log10 3.2 aerobic CFU/cm2 for pork carcasses. The R-mATP assay requires approximately 5 min to complete, including sampling. Given the rapidity and accuracy of the assay, processors interested in monitoring critical control points in the slaughter process could potentially use the R-mATP assay to monitor microbiological prevention and intervention procedures for minimizing carcass contamination.

Original languageEnglish (US)
Pages (from-to)770-775
Number of pages6
JournalJournal of Food Protection
Volume58
Issue number7
DOIs
StatePublished - Jan 1 1995

Fingerprint

pig carcasses
beef carcasses
bioluminescence
Adenosine Triphosphate
assays
plate count
critical control points
Statistical Models
bacterial contamination
monitoring
microbial contamination
Red Meat
statistical models
Swine
pork
slaughter
beef
Bacteria
sampling
swine

All Science Journal Classification (ASJC) codes

  • Food Science
  • Microbiology

Cite this

Siragusa, Gregory R. ; Cutter, Catherine Nettles ; Dorsa, Warren J. ; Koohmaraie, Mohammad. / Use of a rapid microbial ATP bioluminescence assay to detect contamination on beef and pork carcasses. In: Journal of Food Protection. 1995 ; Vol. 58, No. 7. pp. 770-775.
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Use of a rapid microbial ATP bioluminescence assay to detect contamination on beef and pork carcasses. / Siragusa, Gregory R.; Cutter, Catherine Nettles; Dorsa, Warren J.; Koohmaraie, Mohammad.

In: Journal of Food Protection, Vol. 58, No. 7, 01.01.1995, p. 770-775.

Research output: Contribution to journalArticle

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