Use of a ''super-child'' approach to assess the vitamin a equivalence of Moringa oleifera leaves, develop a compartmental model for vitamin a kinetics, and estimate vitamin a total body stores in young Mexican children

Veronica Lopez-Teros, Jennifer Ford, Michael H. Green, Guangwen Tang, Michael A. Grusak, Luis Quihui-Cota, Tawanda Muzhingi, Mariela Paz-Cassini, Humberto Astiazaran-Garcia

Research output: Contribution to journalArticle

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Abstract

Background: Worldwide, an estimated 250 million children < 5 y old are vitamin A (VA) deficient. In Mexico, despite ongoing efforts to reduce VA deficiency, it remains an important public health problem; thus, food-based interventions that increase the availability and consumption of provitamin A-rich foods should be considered. Objective: The objectives were to assess the VA equivalence of 2H-labeled Moringa oleifera (MO) leaves and to estimate both total body stores (TBS) of VA and plasma retinol kinetics in young Mexican children. Methods: β-Carotene was intrinsically labeled by growing MO plants in a 2H2O nutrient solution. Fifteen well-nourished children (17-35 mo old) consumed puréed MO leaves (1 mg β-carotene) and a reference dose of [13C10]retinyl acetate (1 mg) in oil. Blood (2 samples/child) was collected 10 times (2 or 3 children each time) over 35 d. The bioefficacy of MO leaves was calculated from areas under the composite ''super-child'' plasma isotope response curves, and MO VA equivalence was estimated through the use of these values; a compartmental model was developed to predict VA TBS and retinol kinetics through the use of composite plasma [13C10]retinol data. TBS were also estimated with isotope dilution. Results: The relative bioefficacy of β-carotene retinol activity equivalents fromMOwas 28%; VA equivalencewas 3.3:1 byweight (0.56 μmol retinol:1 μmol β-carotene). Kinetics of plasma retinol indicate more rapid plasma appearance and turnover and more extensive recycling in these children than are observed in adults. Model-predicted mean TBS (823 μmol) was similar to values predicted using a retinol isotope dilution equation applied to data from 3 to 6 d after dosing (mean ± SD: 832 ± 176 μmol; n = 7). Conclusions: The super-child approach can be used to estimate population carotenoid bioefficacy and VA equivalence, VA status, and parameters of retinol metabolism from a composite data set. Our results provide initial estimates of retinol kinetics in well-nourished young children with adequate VA stores and demonstrate that MO leaves may be an important source of VA.

Original languageEnglish (US)
Pages (from-to)2356-2363
Number of pages8
JournalJournal of Nutrition
Volume147
Issue number12
DOIs
StatePublished - Dec 1 2017

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Moringa oleifera
Vitamin A
Vitamins
Carotenoids
Isotopes

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Lopez-Teros, Veronica ; Ford, Jennifer ; Green, Michael H. ; Tang, Guangwen ; Grusak, Michael A. ; Quihui-Cota, Luis ; Muzhingi, Tawanda ; Paz-Cassini, Mariela ; Astiazaran-Garcia, Humberto. / Use of a ''super-child'' approach to assess the vitamin a equivalence of Moringa oleifera leaves, develop a compartmental model for vitamin a kinetics, and estimate vitamin a total body stores in young Mexican children. In: Journal of Nutrition. 2017 ; Vol. 147, No. 12. pp. 2356-2363.
@article{c046155013f54529a69125f3ffc346c3,
title = "Use of a ''super-child'' approach to assess the vitamin a equivalence of Moringa oleifera leaves, develop a compartmental model for vitamin a kinetics, and estimate vitamin a total body stores in young Mexican children",
abstract = "Background: Worldwide, an estimated 250 million children < 5 y old are vitamin A (VA) deficient. In Mexico, despite ongoing efforts to reduce VA deficiency, it remains an important public health problem; thus, food-based interventions that increase the availability and consumption of provitamin A-rich foods should be considered. Objective: The objectives were to assess the VA equivalence of 2H-labeled Moringa oleifera (MO) leaves and to estimate both total body stores (TBS) of VA and plasma retinol kinetics in young Mexican children. Methods: β-Carotene was intrinsically labeled by growing MO plants in a 2H2O nutrient solution. Fifteen well-nourished children (17-35 mo old) consumed pur{\'e}ed MO leaves (1 mg β-carotene) and a reference dose of [13C10]retinyl acetate (1 mg) in oil. Blood (2 samples/child) was collected 10 times (2 or 3 children each time) over 35 d. The bioefficacy of MO leaves was calculated from areas under the composite ''super-child'' plasma isotope response curves, and MO VA equivalence was estimated through the use of these values; a compartmental model was developed to predict VA TBS and retinol kinetics through the use of composite plasma [13C10]retinol data. TBS were also estimated with isotope dilution. Results: The relative bioefficacy of β-carotene retinol activity equivalents fromMOwas 28{\%}; VA equivalencewas 3.3:1 byweight (0.56 μmol retinol:1 μmol β-carotene). Kinetics of plasma retinol indicate more rapid plasma appearance and turnover and more extensive recycling in these children than are observed in adults. Model-predicted mean TBS (823 μmol) was similar to values predicted using a retinol isotope dilution equation applied to data from 3 to 6 d after dosing (mean ± SD: 832 ± 176 μmol; n = 7). Conclusions: The super-child approach can be used to estimate population carotenoid bioefficacy and VA equivalence, VA status, and parameters of retinol metabolism from a composite data set. Our results provide initial estimates of retinol kinetics in well-nourished young children with adequate VA stores and demonstrate that MO leaves may be an important source of VA.",
author = "Veronica Lopez-Teros and Jennifer Ford and Green, {Michael H.} and Guangwen Tang and Grusak, {Michael A.} and Luis Quihui-Cota and Tawanda Muzhingi and Mariela Paz-Cassini and Humberto Astiazaran-Garcia",
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Use of a ''super-child'' approach to assess the vitamin a equivalence of Moringa oleifera leaves, develop a compartmental model for vitamin a kinetics, and estimate vitamin a total body stores in young Mexican children. / Lopez-Teros, Veronica; Ford, Jennifer; Green, Michael H.; Tang, Guangwen; Grusak, Michael A.; Quihui-Cota, Luis; Muzhingi, Tawanda; Paz-Cassini, Mariela; Astiazaran-Garcia, Humberto.

In: Journal of Nutrition, Vol. 147, No. 12, 01.12.2017, p. 2356-2363.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Use of a ''super-child'' approach to assess the vitamin a equivalence of Moringa oleifera leaves, develop a compartmental model for vitamin a kinetics, and estimate vitamin a total body stores in young Mexican children

AU - Lopez-Teros, Veronica

AU - Ford, Jennifer

AU - Green, Michael H.

AU - Tang, Guangwen

AU - Grusak, Michael A.

AU - Quihui-Cota, Luis

AU - Muzhingi, Tawanda

AU - Paz-Cassini, Mariela

AU - Astiazaran-Garcia, Humberto

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Background: Worldwide, an estimated 250 million children < 5 y old are vitamin A (VA) deficient. In Mexico, despite ongoing efforts to reduce VA deficiency, it remains an important public health problem; thus, food-based interventions that increase the availability and consumption of provitamin A-rich foods should be considered. Objective: The objectives were to assess the VA equivalence of 2H-labeled Moringa oleifera (MO) leaves and to estimate both total body stores (TBS) of VA and plasma retinol kinetics in young Mexican children. Methods: β-Carotene was intrinsically labeled by growing MO plants in a 2H2O nutrient solution. Fifteen well-nourished children (17-35 mo old) consumed puréed MO leaves (1 mg β-carotene) and a reference dose of [13C10]retinyl acetate (1 mg) in oil. Blood (2 samples/child) was collected 10 times (2 or 3 children each time) over 35 d. The bioefficacy of MO leaves was calculated from areas under the composite ''super-child'' plasma isotope response curves, and MO VA equivalence was estimated through the use of these values; a compartmental model was developed to predict VA TBS and retinol kinetics through the use of composite plasma [13C10]retinol data. TBS were also estimated with isotope dilution. Results: The relative bioefficacy of β-carotene retinol activity equivalents fromMOwas 28%; VA equivalencewas 3.3:1 byweight (0.56 μmol retinol:1 μmol β-carotene). Kinetics of plasma retinol indicate more rapid plasma appearance and turnover and more extensive recycling in these children than are observed in adults. Model-predicted mean TBS (823 μmol) was similar to values predicted using a retinol isotope dilution equation applied to data from 3 to 6 d after dosing (mean ± SD: 832 ± 176 μmol; n = 7). Conclusions: The super-child approach can be used to estimate population carotenoid bioefficacy and VA equivalence, VA status, and parameters of retinol metabolism from a composite data set. Our results provide initial estimates of retinol kinetics in well-nourished young children with adequate VA stores and demonstrate that MO leaves may be an important source of VA.

AB - Background: Worldwide, an estimated 250 million children < 5 y old are vitamin A (VA) deficient. In Mexico, despite ongoing efforts to reduce VA deficiency, it remains an important public health problem; thus, food-based interventions that increase the availability and consumption of provitamin A-rich foods should be considered. Objective: The objectives were to assess the VA equivalence of 2H-labeled Moringa oleifera (MO) leaves and to estimate both total body stores (TBS) of VA and plasma retinol kinetics in young Mexican children. Methods: β-Carotene was intrinsically labeled by growing MO plants in a 2H2O nutrient solution. Fifteen well-nourished children (17-35 mo old) consumed puréed MO leaves (1 mg β-carotene) and a reference dose of [13C10]retinyl acetate (1 mg) in oil. Blood (2 samples/child) was collected 10 times (2 or 3 children each time) over 35 d. The bioefficacy of MO leaves was calculated from areas under the composite ''super-child'' plasma isotope response curves, and MO VA equivalence was estimated through the use of these values; a compartmental model was developed to predict VA TBS and retinol kinetics through the use of composite plasma [13C10]retinol data. TBS were also estimated with isotope dilution. Results: The relative bioefficacy of β-carotene retinol activity equivalents fromMOwas 28%; VA equivalencewas 3.3:1 byweight (0.56 μmol retinol:1 μmol β-carotene). Kinetics of plasma retinol indicate more rapid plasma appearance and turnover and more extensive recycling in these children than are observed in adults. Model-predicted mean TBS (823 μmol) was similar to values predicted using a retinol isotope dilution equation applied to data from 3 to 6 d after dosing (mean ± SD: 832 ± 176 μmol; n = 7). Conclusions: The super-child approach can be used to estimate population carotenoid bioefficacy and VA equivalence, VA status, and parameters of retinol metabolism from a composite data set. Our results provide initial estimates of retinol kinetics in well-nourished young children with adequate VA stores and demonstrate that MO leaves may be an important source of VA.

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JF - Journal of Nutrition

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