Use of a third-generation perfluorocarbon for preservation of rat DCD liver grafts

Dmitri Bezinover, Saravanan Ramamoorthy, Todahiro Uemura, Zakiyah Kadry, Patrick M. McQuillan, Berend Mets, Octavio Falcucci, Sharon Rannels, Victor Ruiz-Velasco, Bruce Spiess, John Liang, Haresh Mani, Xi Lou, Piotr K. Janicki

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Cold storage in any of the commonly used preservation solutions is not always adequate for donation after cardiac death (DCD) liver grafts due to prolonged warm ischemic time. In this study, we used a third-generation perfluorocarbon (PFC), Oxycyte, for DCD liver graft preservation in a rat model. Materials and Methods: Twenty-eight rats (14 in each group) were used. Thirty minutes after cardiopulmonary arrest, livers were harvested and flushed with a cold and pre-oxygenated solution of either University of Wisconsin (UW) or UW + 20% PFC. After 8 h of cold preservation in either of the investigated solutions, liver graft specimens were analyzed for evidence of ischemic injury. Hemotoxylin and eosin staining (H and E), as well as immunohistochemical analysis with anti-cleaved caspase 3 antibody, was performed. Levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the preservation solution were analyzed at 1 and 8 h during preservation. Results: In the PFC group, the degree of cell congestion, vacuolization and necrosis were all significantly less than in the UW group (P = 0.002-0.004). The number of cells with a positive cleaved caspase 3 antibody reaction was reduced by about 50% in comparison with the UW group (P < 0.006). The AST level in the PFC group was significantly less than in the UW group after 8 h of preservation (P < 0.048). Conclusion: The addition of PFC to UW solution significantly decreases the degree of histologic damage in rat DCD liver grafts. This preservation strategy can be potentially helpful for organ preservation after prolonged warm ischemia.

Original languageEnglish (US)
Pages (from-to)131-137
Number of pages7
JournalJournal of Surgical Research
Volume175
Issue number1
DOIs
StatePublished - Jun 1 2012

Fingerprint

Fluorocarbons
Transplants
Liver
Warm Ischemia
Aspartate Aminotransferases
Caspase 3
Organ Preservation
Antibodies
Hematoxylin
Eosine Yellowish-(YS)
Heart Arrest
Alanine Transaminase
Necrosis
Cell Count
Staining and Labeling
Wounds and Injuries

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Bezinover, Dmitri ; Ramamoorthy, Saravanan ; Uemura, Todahiro ; Kadry, Zakiyah ; McQuillan, Patrick M. ; Mets, Berend ; Falcucci, Octavio ; Rannels, Sharon ; Ruiz-Velasco, Victor ; Spiess, Bruce ; Liang, John ; Mani, Haresh ; Lou, Xi ; Janicki, Piotr K. / Use of a third-generation perfluorocarbon for preservation of rat DCD liver grafts. In: Journal of Surgical Research. 2012 ; Vol. 175, No. 1. pp. 131-137.
@article{0c442398d892422683713afdfd8130e6,
title = "Use of a third-generation perfluorocarbon for preservation of rat DCD liver grafts",
abstract = "Background: Cold storage in any of the commonly used preservation solutions is not always adequate for donation after cardiac death (DCD) liver grafts due to prolonged warm ischemic time. In this study, we used a third-generation perfluorocarbon (PFC), Oxycyte, for DCD liver graft preservation in a rat model. Materials and Methods: Twenty-eight rats (14 in each group) were used. Thirty minutes after cardiopulmonary arrest, livers were harvested and flushed with a cold and pre-oxygenated solution of either University of Wisconsin (UW) or UW + 20{\%} PFC. After 8 h of cold preservation in either of the investigated solutions, liver graft specimens were analyzed for evidence of ischemic injury. Hemotoxylin and eosin staining (H and E), as well as immunohistochemical analysis with anti-cleaved caspase 3 antibody, was performed. Levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the preservation solution were analyzed at 1 and 8 h during preservation. Results: In the PFC group, the degree of cell congestion, vacuolization and necrosis were all significantly less than in the UW group (P = 0.002-0.004). The number of cells with a positive cleaved caspase 3 antibody reaction was reduced by about 50{\%} in comparison with the UW group (P < 0.006). The AST level in the PFC group was significantly less than in the UW group after 8 h of preservation (P < 0.048). Conclusion: The addition of PFC to UW solution significantly decreases the degree of histologic damage in rat DCD liver grafts. This preservation strategy can be potentially helpful for organ preservation after prolonged warm ischemia.",
author = "Dmitri Bezinover and Saravanan Ramamoorthy and Todahiro Uemura and Zakiyah Kadry and McQuillan, {Patrick M.} and Berend Mets and Octavio Falcucci and Sharon Rannels and Victor Ruiz-Velasco and Bruce Spiess and John Liang and Haresh Mani and Xi Lou and Janicki, {Piotr K.}",
year = "2012",
month = "6",
day = "1",
doi = "10.1016/j.jss.2011.02.046",
language = "English (US)",
volume = "175",
pages = "131--137",
journal = "Journal of Surgical Research",
issn = "0022-4804",
publisher = "Academic Press Inc.",
number = "1",

}

Use of a third-generation perfluorocarbon for preservation of rat DCD liver grafts. / Bezinover, Dmitri; Ramamoorthy, Saravanan; Uemura, Todahiro; Kadry, Zakiyah; McQuillan, Patrick M.; Mets, Berend; Falcucci, Octavio; Rannels, Sharon; Ruiz-Velasco, Victor; Spiess, Bruce; Liang, John; Mani, Haresh; Lou, Xi; Janicki, Piotr K.

In: Journal of Surgical Research, Vol. 175, No. 1, 01.06.2012, p. 131-137.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Use of a third-generation perfluorocarbon for preservation of rat DCD liver grafts

AU - Bezinover, Dmitri

AU - Ramamoorthy, Saravanan

AU - Uemura, Todahiro

AU - Kadry, Zakiyah

AU - McQuillan, Patrick M.

AU - Mets, Berend

AU - Falcucci, Octavio

AU - Rannels, Sharon

AU - Ruiz-Velasco, Victor

AU - Spiess, Bruce

AU - Liang, John

AU - Mani, Haresh

AU - Lou, Xi

AU - Janicki, Piotr K.

PY - 2012/6/1

Y1 - 2012/6/1

N2 - Background: Cold storage in any of the commonly used preservation solutions is not always adequate for donation after cardiac death (DCD) liver grafts due to prolonged warm ischemic time. In this study, we used a third-generation perfluorocarbon (PFC), Oxycyte, for DCD liver graft preservation in a rat model. Materials and Methods: Twenty-eight rats (14 in each group) were used. Thirty minutes after cardiopulmonary arrest, livers were harvested and flushed with a cold and pre-oxygenated solution of either University of Wisconsin (UW) or UW + 20% PFC. After 8 h of cold preservation in either of the investigated solutions, liver graft specimens were analyzed for evidence of ischemic injury. Hemotoxylin and eosin staining (H and E), as well as immunohistochemical analysis with anti-cleaved caspase 3 antibody, was performed. Levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the preservation solution were analyzed at 1 and 8 h during preservation. Results: In the PFC group, the degree of cell congestion, vacuolization and necrosis were all significantly less than in the UW group (P = 0.002-0.004). The number of cells with a positive cleaved caspase 3 antibody reaction was reduced by about 50% in comparison with the UW group (P < 0.006). The AST level in the PFC group was significantly less than in the UW group after 8 h of preservation (P < 0.048). Conclusion: The addition of PFC to UW solution significantly decreases the degree of histologic damage in rat DCD liver grafts. This preservation strategy can be potentially helpful for organ preservation after prolonged warm ischemia.

AB - Background: Cold storage in any of the commonly used preservation solutions is not always adequate for donation after cardiac death (DCD) liver grafts due to prolonged warm ischemic time. In this study, we used a third-generation perfluorocarbon (PFC), Oxycyte, for DCD liver graft preservation in a rat model. Materials and Methods: Twenty-eight rats (14 in each group) were used. Thirty minutes after cardiopulmonary arrest, livers were harvested and flushed with a cold and pre-oxygenated solution of either University of Wisconsin (UW) or UW + 20% PFC. After 8 h of cold preservation in either of the investigated solutions, liver graft specimens were analyzed for evidence of ischemic injury. Hemotoxylin and eosin staining (H and E), as well as immunohistochemical analysis with anti-cleaved caspase 3 antibody, was performed. Levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the preservation solution were analyzed at 1 and 8 h during preservation. Results: In the PFC group, the degree of cell congestion, vacuolization and necrosis were all significantly less than in the UW group (P = 0.002-0.004). The number of cells with a positive cleaved caspase 3 antibody reaction was reduced by about 50% in comparison with the UW group (P < 0.006). The AST level in the PFC group was significantly less than in the UW group after 8 h of preservation (P < 0.048). Conclusion: The addition of PFC to UW solution significantly decreases the degree of histologic damage in rat DCD liver grafts. This preservation strategy can be potentially helpful for organ preservation after prolonged warm ischemia.

UR - http://www.scopus.com/inward/record.url?scp=84860339215&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84860339215&partnerID=8YFLogxK

U2 - 10.1016/j.jss.2011.02.046

DO - 10.1016/j.jss.2011.02.046

M3 - Article

C2 - 21543088

AN - SCOPUS:84860339215

VL - 175

SP - 131

EP - 137

JO - Journal of Surgical Research

JF - Journal of Surgical Research

SN - 0022-4804

IS - 1

ER -