Abstract
This overview unit discusses fluorescence spectroscopy as a tool for studying RNA folding. Ribozymes and oligonucleotides can be labeled with a fluorescent probe and analyzed to give information about both slow and fast kinetic processes with real-time data acquisition. The unit discusses the advantages and disadvantages of various pendant probes and nucleotide analogs, the analytical methods that can be used, instrument setup, control experiments, and a variety of kinetic experiments that can be performed, such as determination of rate constants.
| Original language | English (US) |
|---|---|
| Journal | Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage ... [et al.] |
| Volume | Chapter 11 |
| State | Published - Jan 1 2002 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Organic Chemistry
Cite this
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Use of fluorescence spectroscopy to elucidate RNA folding pathways. / Bevilacqua, Philip C.; Turner, Douglas H.
In: Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage ... [et al.], Vol. Chapter 11, 01.01.2002.Research output: Contribution to journal › Article
TY - JOUR
T1 - Use of fluorescence spectroscopy to elucidate RNA folding pathways.
AU - Bevilacqua, Philip C.
AU - Turner, Douglas H.
PY - 2002/1/1
Y1 - 2002/1/1
N2 - This overview unit discusses fluorescence spectroscopy as a tool for studying RNA folding. Ribozymes and oligonucleotides can be labeled with a fluorescent probe and analyzed to give information about both slow and fast kinetic processes with real-time data acquisition. The unit discusses the advantages and disadvantages of various pendant probes and nucleotide analogs, the analytical methods that can be used, instrument setup, control experiments, and a variety of kinetic experiments that can be performed, such as determination of rate constants.
AB - This overview unit discusses fluorescence spectroscopy as a tool for studying RNA folding. Ribozymes and oligonucleotides can be labeled with a fluorescent probe and analyzed to give information about both slow and fast kinetic processes with real-time data acquisition. The unit discusses the advantages and disadvantages of various pendant probes and nucleotide analogs, the analytical methods that can be used, instrument setup, control experiments, and a variety of kinetic experiments that can be performed, such as determination of rate constants.
UR - http://www.scopus.com/inward/record.url?scp=44249114590&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=44249114590&partnerID=8YFLogxK
M3 - Article
VL - Chapter 11
JO - Current Protocols in Nucleic Acid Chemistry
JF - Current Protocols in Nucleic Acid Chemistry
SN - 1934-9270
ER -