Abstract
This overview unit discusses fluorescence spectroscopy as a tool for studying RNA folding. Ribozymes and oligonucleotides can be labeled with a fluorescent probe and analyzed to give information about both slow and fast kinetic processes with real-time data acquisition. The unit discusses the advantages and disadvantages of various pendant probes and nucleotide analogs, the analytical methods that can be used, instrument setup, control experiments, and a variety of kinetic experiments that can be performed, such as determination of rate constants.
Original language | English (US) |
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Journal | Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage ... [et al.] |
Volume | Chapter 11 |
State | Published - Jan 1 2002 |
All Science Journal Classification (ASJC) codes
- Biochemistry
- Organic Chemistry