Use of fluorescence spectroscopy to elucidate RNA folding pathways.

Philip C. Bevilacqua, Douglas H. Turner

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

This overview unit discusses fluorescence spectroscopy as a tool for studying RNA folding. Ribozymes and oligonucleotides can be labeled with a fluorescent probe and analyzed to give information about both slow and fast kinetic processes with real-time data acquisition. The unit discusses the advantages and disadvantages of various pendant probes and nucleotide analogs, the analytical methods that can be used, instrument setup, control experiments, and a variety of kinetic experiments that can be performed, such as determination of rate constants.

Original languageEnglish (US)
JournalCurrent protocols in nucleic acid chemistry / edited by Serge L. Beaucage ... [et al.]
VolumeChapter 11
StatePublished - Jan 1 2002

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Organic Chemistry

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