Use of fluorescence spectroscopy to elucidate RNA folding pathways.

Philip C. Bevilacqua, Douglas H. Turner

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This overview unit discusses fluorescence spectroscopy as a tool for studying RNA folding. Ribozymes and oligonucleotides can be labeled with a fluorescent probe and analyzed to give information about both slow and fast kinetic processes with real-time data acquisition. The unit discusses the advantages and disadvantages of various pendant probes and nucleotide analogs, the analytical methods that can be used, instrument setup, control experiments, and a variety of kinetic experiments that can be performed, such as determination of rate constants.

Original languageEnglish (US)
JournalCurrent protocols in nucleic acid chemistry / edited by Serge L. Beaucage ... [et al.]
VolumeChapter 11
StatePublished - Jan 1 2002

Fingerprint

RNA Folding
Fluorescence Spectrometry
Fluorescence spectroscopy
RNA
Catalytic RNA
Kinetics
Fluorescent Dyes
Oligonucleotides
Rate constants
Data acquisition
Nucleotides
Experiments

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Organic Chemistry

Cite this

@article{a1d9312f61e34e9dbf8565c463faffae,
title = "Use of fluorescence spectroscopy to elucidate RNA folding pathways.",
abstract = "This overview unit discusses fluorescence spectroscopy as a tool for studying RNA folding. Ribozymes and oligonucleotides can be labeled with a fluorescent probe and analyzed to give information about both slow and fast kinetic processes with real-time data acquisition. The unit discusses the advantages and disadvantages of various pendant probes and nucleotide analogs, the analytical methods that can be used, instrument setup, control experiments, and a variety of kinetic experiments that can be performed, such as determination of rate constants.",
author = "Bevilacqua, {Philip C.} and Turner, {Douglas H.}",
year = "2002",
month = "1",
day = "1",
language = "English (US)",
volume = "Chapter 11",
journal = "Current Protocols in Nucleic Acid Chemistry",
issn = "1934-9270",
publisher = "John Wiley and Sons Inc.",

}

TY - JOUR

T1 - Use of fluorescence spectroscopy to elucidate RNA folding pathways.

AU - Bevilacqua, Philip C.

AU - Turner, Douglas H.

PY - 2002/1/1

Y1 - 2002/1/1

N2 - This overview unit discusses fluorescence spectroscopy as a tool for studying RNA folding. Ribozymes and oligonucleotides can be labeled with a fluorescent probe and analyzed to give information about both slow and fast kinetic processes with real-time data acquisition. The unit discusses the advantages and disadvantages of various pendant probes and nucleotide analogs, the analytical methods that can be used, instrument setup, control experiments, and a variety of kinetic experiments that can be performed, such as determination of rate constants.

AB - This overview unit discusses fluorescence spectroscopy as a tool for studying RNA folding. Ribozymes and oligonucleotides can be labeled with a fluorescent probe and analyzed to give information about both slow and fast kinetic processes with real-time data acquisition. The unit discusses the advantages and disadvantages of various pendant probes and nucleotide analogs, the analytical methods that can be used, instrument setup, control experiments, and a variety of kinetic experiments that can be performed, such as determination of rate constants.

UR - http://www.scopus.com/inward/record.url?scp=44249114590&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=44249114590&partnerID=8YFLogxK

M3 - Article

VL - Chapter 11

JO - Current Protocols in Nucleic Acid Chemistry

JF - Current Protocols in Nucleic Acid Chemistry

SN - 1934-9270

ER -