Use of microarray analysis to study gene expression in the avian epiphyseal growth plate

Maria Horvat-Gordon, C. A. Praul, Ramesh Ramachandran, Paul Allen Bartell, R. M. Leach

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Longitudinal bone growth depends upon the execution of an intricate series of cellular activities by epiphyseal growth plate chondrocytes. In order to better understand these coordinated events, microarray analysis was used to compare gene expression in chondrocytes isolated from the proliferative and hypertrophic zones of the avian growth plate. RT-PCR was used to confirm the identity of a select number of genes. The expression of 745 genes was found to differ 3-fold or greater at the 0.05 level of probability. Transferrin was the most highly up-regulated (321-fold) gene associated with chondrocyte hypertrophy. Immunohistochemistry localized this peptide adjacent to the penetrating blood vessels in the growth plate of 3-week-old chicks. Fibulin, OC-116, DMP-1 and PHEX were among the expanded number of genes associated with extracellular matrix metabolism. The presence of NELL2, ATOH8 and PLEXIN suggests a neuronal involvement in growth plate physiology. In addition, the expression of a large number of genes associated with angiogenesis and cellular stress was up-regulated. These processes are important to the physiology and survival of chondrocytes in the unique and stressful environment of the epiphyseal growth plate.

Original languageEnglish (US)
Pages (from-to)12-23
Number of pages12
JournalComparative Biochemistry and Physiology - Part D: Genomics and Proteomics
Volume5
Issue number1
DOIs
StatePublished - Mar 1 2010

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Growth Plate
Microarray Analysis
Microarrays
Gene expression
Gene Expression
Genes
Chondrocytes
Physiology
Blood vessels
Transferrin
Metabolism
Bone Development
Bone
Hypertrophy
Extracellular Matrix
Blood Vessels
Immunohistochemistry
Peptides
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Molecular Biology
  • Genetics

Cite this

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abstract = "Longitudinal bone growth depends upon the execution of an intricate series of cellular activities by epiphyseal growth plate chondrocytes. In order to better understand these coordinated events, microarray analysis was used to compare gene expression in chondrocytes isolated from the proliferative and hypertrophic zones of the avian growth plate. RT-PCR was used to confirm the identity of a select number of genes. The expression of 745 genes was found to differ 3-fold or greater at the 0.05 level of probability. Transferrin was the most highly up-regulated (321-fold) gene associated with chondrocyte hypertrophy. Immunohistochemistry localized this peptide adjacent to the penetrating blood vessels in the growth plate of 3-week-old chicks. Fibulin, OC-116, DMP-1 and PHEX were among the expanded number of genes associated with extracellular matrix metabolism. The presence of NELL2, ATOH8 and PLEXIN suggests a neuronal involvement in growth plate physiology. In addition, the expression of a large number of genes associated with angiogenesis and cellular stress was up-regulated. These processes are important to the physiology and survival of chondrocytes in the unique and stressful environment of the epiphyseal growth plate.",
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Use of microarray analysis to study gene expression in the avian epiphyseal growth plate. / Horvat-Gordon, Maria; Praul, C. A.; Ramachandran, Ramesh; Bartell, Paul Allen; Leach, R. M.

In: Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, Vol. 5, No. 1, 01.03.2010, p. 12-23.

Research output: Contribution to journalArticle

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