Degenerate PCR probes were used to amplify gene fragments encoding the catalytic domain of σ54-dependent transcription activators. The procedure should be widely applicable, as it recovered both known and novel gene fragments: 5 from Rhizobium meliloti, 13 from Myxococcus xanthus, and 3 from Bacillus subtilis. No fragments were obtained from Synechococcus sp. strain PCC 7002 or Saccharomyces cerevisiae.
|Original language||English (US)|
|Number of pages||4|
|Journal||Journal of bacteriology|
|Publication status||Published - Jul 19 1996|
All Science Journal Classification (ASJC) codes
- Molecular Biology