Use of PCR to isolate genes encoding σ54-dependent activators from diverse bacteria

R. Ilene Kaufman, B. Tracy Nixon

Research output: Contribution to journalArticle

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Abstract

Degenerate PCR probes were used to amplify gene fragments encoding the catalytic domain of σ54-dependent transcription activators. The procedure should be widely applicable, as it recovered both known and novel gene fragments: 5 from Rhizobium meliloti, 13 from Myxococcus xanthus, and 3 from Bacillus subtilis. No fragments were obtained from Synechococcus sp. strain PCC 7002 or Saccharomyces cerevisiae.

Original languageEnglish (US)
Pages (from-to)3967-3970
Number of pages4
JournalJournal of bacteriology
Volume178
Issue number13
Publication statusPublished - Jul 19 1996

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All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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