Use of RNA interference to dissect defense-signaling pathways in rice.

Chuansheng Mei, Xiangjun Zhou, Yinong Yang

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The RNA interference (RNAi) technique is a powerful tool to suppress gene expression and has been widely used for functional discovery of eukaryotic genes. To dissect defense-signaling pathways in rice, it is important to generate a series of rice mutant lines deficient in or insensitive to major signal molecules such as jasmonic acid and ethylene. Here we describe an RNAi protocol for generating and characterizing transgenic gene-silencing lines defective in rice jasmonic acid signaling. The RNAi technique should be useful for effective suppression of host genes encoding signaling components and facilitating the dissection of defense signal pathways in rice.

Original languageEnglish (US)
Pages (from-to)161-171
Number of pages11
JournalMethods in Molecular Biology
Volume354
StatePublished - 2007

Fingerprint

RNA Interference
Genetic Association Studies
Gene Silencing
Dissection
Signal Transduction
Gene Expression
Oryza
Genes
jasmonic acid

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

@article{85dbdc8b1f3b40a4b47ee5216c1786d4,
title = "Use of RNA interference to dissect defense-signaling pathways in rice.",
abstract = "The RNA interference (RNAi) technique is a powerful tool to suppress gene expression and has been widely used for functional discovery of eukaryotic genes. To dissect defense-signaling pathways in rice, it is important to generate a series of rice mutant lines deficient in or insensitive to major signal molecules such as jasmonic acid and ethylene. Here we describe an RNAi protocol for generating and characterizing transgenic gene-silencing lines defective in rice jasmonic acid signaling. The RNAi technique should be useful for effective suppression of host genes encoding signaling components and facilitating the dissection of defense signal pathways in rice.",
author = "Chuansheng Mei and Xiangjun Zhou and Yinong Yang",
year = "2007",
language = "English (US)",
volume = "354",
pages = "161--171",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

Use of RNA interference to dissect defense-signaling pathways in rice. / Mei, Chuansheng; Zhou, Xiangjun; Yang, Yinong.

In: Methods in Molecular Biology, Vol. 354, 2007, p. 161-171.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Use of RNA interference to dissect defense-signaling pathways in rice.

AU - Mei, Chuansheng

AU - Zhou, Xiangjun

AU - Yang, Yinong

PY - 2007

Y1 - 2007

N2 - The RNA interference (RNAi) technique is a powerful tool to suppress gene expression and has been widely used for functional discovery of eukaryotic genes. To dissect defense-signaling pathways in rice, it is important to generate a series of rice mutant lines deficient in or insensitive to major signal molecules such as jasmonic acid and ethylene. Here we describe an RNAi protocol for generating and characterizing transgenic gene-silencing lines defective in rice jasmonic acid signaling. The RNAi technique should be useful for effective suppression of host genes encoding signaling components and facilitating the dissection of defense signal pathways in rice.

AB - The RNA interference (RNAi) technique is a powerful tool to suppress gene expression and has been widely used for functional discovery of eukaryotic genes. To dissect defense-signaling pathways in rice, it is important to generate a series of rice mutant lines deficient in or insensitive to major signal molecules such as jasmonic acid and ethylene. Here we describe an RNAi protocol for generating and characterizing transgenic gene-silencing lines defective in rice jasmonic acid signaling. The RNAi technique should be useful for effective suppression of host genes encoding signaling components and facilitating the dissection of defense signal pathways in rice.

UR - http://www.scopus.com/inward/record.url?scp=39049176996&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=39049176996&partnerID=8YFLogxK

M3 - Article

VL - 354

SP - 161

EP - 171

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -