Use of the escherichia coll ssb gene to prevent bioreactor takeover by ptasmidles ceils

Ronald D. Porter, Stuart Black, Sachin Pannuri, Alfred Carlson

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Reactor takeover by plasmidless cells is i major problem encountered when produc ing proteins from plasmid-borne genes ii genetically engineered bacteria. We have approached this problem by deleting the essential ssb gene from the Escherichia col chromosome and placing it on a plasmid Plasmidless cells do not accumulate ever after growing such strains under non-se lective continuous culture conditions foi extended periods of time. Other ssb-con taining plasmids can be readily intro duced into this E. coli strain by a plasmid displacement technique. Using this sys tem, we have achieved very high levels o: 3-lactamase production in continuous cul ture without selective pressure.

Original languageEnglish (US)
Pages (from-to)47-51
Number of pages5
JournalBio/Technology
Volume8
Issue number1
DOIs
StatePublished - Jan 1 1990

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Escherichia
Bioreactors
Plasmids
Genes
Chromosomes
Escherichia coli
Bacteria
Proteins
Essential Genes

All Science Journal Classification (ASJC) codes

  • Biotechnology

Cite this

Porter, Ronald D. ; Black, Stuart ; Pannuri, Sachin ; Carlson, Alfred. / Use of the escherichia coll ssb gene to prevent bioreactor takeover by ptasmidles ceils. In: Bio/Technology. 1990 ; Vol. 8, No. 1. pp. 47-51.
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Use of the escherichia coll ssb gene to prevent bioreactor takeover by ptasmidles ceils. / Porter, Ronald D.; Black, Stuart; Pannuri, Sachin; Carlson, Alfred.

In: Bio/Technology, Vol. 8, No. 1, 01.01.1990, p. 47-51.

Research output: Contribution to journalArticle

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