Use of the escherichia coll ssb gene to prevent bioreactor takeover by ptasmidles ceils

Ronald D. Porter, Stuart Black, Sachin Pannuri, Alfred Carlson

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

Reactor takeover by plasmidless cells is i major problem encountered when produc ing proteins from plasmid-borne genes ii genetically engineered bacteria. We have approached this problem by deleting the essential ssb gene from the Escherichia col chromosome and placing it on a plasmid Plasmidless cells do not accumulate ever after growing such strains under non-se lective continuous culture conditions foi extended periods of time. Other ssb-con taining plasmids can be readily intro duced into this E. coli strain by a plasmid displacement technique. Using this sys tem, we have achieved very high levels o: 3-lactamase production in continuous cul ture without selective pressure.

Original languageEnglish (US)
Pages (from-to)47-51
Number of pages5
JournalBio/Technology
Volume8
Issue number1
DOIs
StatePublished - Jan 1 1990

All Science Journal Classification (ASJC) codes

  • Biotechnology

Fingerprint Dive into the research topics of 'Use of the escherichia coll ssb gene to prevent bioreactor takeover by ptasmidles ceils'. Together they form a unique fingerprint.

Cite this