Use of Urinary Biomarkers of Renal Ischemia in a Lamb Preclinical Left Ventricular Assist Device Model

Timothy Cooper, Qing Zhong, Mary Nabity, Gerson Rosenberg, William Weiss

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Evaluation of thrombogenicity is a critical component in the preclinical testing and development of blood pumps. Left ventricular assist devices (LVADs), because of their device routing, can produce thromboembolic showers to the kidney resulting in renal cortical ischemia or infarctions. Although postmortem evaluation of renal pathology can confirm ischemic events and infarctions, there are no validated and highly sensitive real-time measures of renal ischemia in the preclinical models. In this article, we report the evaluation of urinary biomarkers of ischemic tubular damage in a lamb preclinical LVAD model. We found that urinary excretion of glutathione-S-transferase-π, heat shock protein 1B, and hepatitis A virus cellular receptor 1 homologue precursor (HAVCR1/kidney injury molecule 1) were upregulated in toxic ischemic renal injury as well as in the immediate postoperative period in an LVAD-implanted lamb. These markers were consistent with both gross and histologic pathology, and proved far more sensitive for renal injury than serum blood urea nitrogen or creatinine concentrations.

Original languageEnglish (US)
Pages (from-to)820-824
Number of pages5
JournalArtificial organs
Volume36
Issue number9
DOIs
StatePublished - Sep 1 2012

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Left ventricular assist devices
Heart-Assist Devices
Biomarkers
Ischemia
Pathology
Kidney
Blood
Poisons
Heat-Shock Proteins
Glutathione Transferase
Viruses
Urea
Creatinine
Infarction
Nitrogen
Pumps
Wounds and Injuries
Proteins
Molecules
Testing

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

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abstract = "Evaluation of thrombogenicity is a critical component in the preclinical testing and development of blood pumps. Left ventricular assist devices (LVADs), because of their device routing, can produce thromboembolic showers to the kidney resulting in renal cortical ischemia or infarctions. Although postmortem evaluation of renal pathology can confirm ischemic events and infarctions, there are no validated and highly sensitive real-time measures of renal ischemia in the preclinical models. In this article, we report the evaluation of urinary biomarkers of ischemic tubular damage in a lamb preclinical LVAD model. We found that urinary excretion of glutathione-S-transferase-π, heat shock protein 1B, and hepatitis A virus cellular receptor 1 homologue precursor (HAVCR1/kidney injury molecule 1) were upregulated in toxic ischemic renal injury as well as in the immediate postoperative period in an LVAD-implanted lamb. These markers were consistent with both gross and histologic pathology, and proved far more sensitive for renal injury than serum blood urea nitrogen or creatinine concentrations.",
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Use of Urinary Biomarkers of Renal Ischemia in a Lamb Preclinical Left Ventricular Assist Device Model. / Cooper, Timothy; Zhong, Qing; Nabity, Mary; Rosenberg, Gerson; Weiss, William.

In: Artificial organs, Vol. 36, No. 9, 01.09.2012, p. 820-824.

Research output: Contribution to journalArticle

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