Using an AraC-based three-hybrid system to detect biocatalysts in vivo

Steven M. Firestine, Frank Salinas, Andrew E. Nixon, Stephen J. Baker, Stephen J. Benkovic

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Recent methods to create large libraries of proteins have greatly advanced the discovery of proteins with novel functions. However, one limitation in the discovery of new biocatalysts is the screening or selection methods employed to find enzymes from these libraries. We have developed a potentially general method termed QUEST (QUerying for Enzymes using the Three-hybrid system), which allows the construction of an easily screened or selected phenotype for, in theory, any type of enzymatic reaction. The method couples the in vive concentration of an enzyme's substrate to changes in the transcriptional level of a reporter operon. Using the arabinose operon activator AraC, we constructed a system capable of detecting the fungal enzyme scytalone dehydratase (SD) in bacteria, and demonstrated its sensitivity and usefulness in library screening.

Original languageEnglish (US)
Pages (from-to)544-547
Number of pages4
JournalNature Biotechnology
Volume18
Issue number5
DOIs
StatePublished - May 2000

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

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