Using imaging ToF-SIMS data to determine the cell wall thickness of fibers in wood

Here, we demonstrate the use of time-of-flight secondary ion mass spectrometry (ToF-SIMS) imaging data for estimation of cell wall thickness in wood samples. Current research in forest biotechnology focuses on transgenic trees with wood properties tailored to specific applications. Appropriate analytical methods to characterize the very heterogeneous wood material are constantly being developed and improved. Chemical imaging ofwood by ToF-SIMS represents an interesting tool for this purpose with many applications. In addition towood chemistry, the impact of specific geneticmodifications onwood anatomy needs to be assessed. Cell wall thickness is an important anatomical parameter that among others is used for assessing biomass accumulation. We developed a strategy to estimate cell wall thickness fromToF-SIMS images and implemented it in the open source programming language 'R'. In brief, randomlines are projected over the black and white mask of a ToF-SIMS image, and length values of all line sections that cut across a cell wall are collected. After enough iteration, the shortest values of the obtained count distribution represent the crossing sections normal to the cell walls, hence cell wall thickness. Compared with conventional light microscopy image analysis, TOF-SIMS data offers many advantages such as submicron resolution and additional spectral information for automated annotation of distinct anatomical features. This work underlines the importance of SIMS imaging for studies of wood chemistry and anatomy and provides a new approach to obtain an important wood anatomical parameter from ToF-SIMS data.