Using miniature osmotic infusion pumps to maintain tritiated thymidine exposure to cells in culture

John Neely, D. A. Hake

Research output: Contribution to journalArticle

Abstract

To provide a constant level of tracer doses of tritiated thymidine to cultured cells during continuous infusion, miniature osmotic infusion pumps were used to provide replacement thymidine. By determining the loss of isotope from the media during nonreplacement, the rate of constant infusion replacement to maintain thymidine levels was calculated. The replacement rates were similar for the three cell lines examined and allowed a standard osmotic pump infusion.

Original languageEnglish (US)
Pages (from-to)536-537
Number of pages2
JournalJournal of Histochemistry and Cytochemistry
Volume30
Issue number6
DOIs
StatePublished - Jan 1 1982

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Infusion Pumps
Thymidine
Cell Culture Techniques
Isotopes
Cultured Cells
Cell Line

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Histology

Cite this

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Using miniature osmotic infusion pumps to maintain tritiated thymidine exposure to cells in culture. / Neely, John; Hake, D. A.

In: Journal of Histochemistry and Cytochemistry, Vol. 30, No. 6, 01.01.1982, p. 536-537.

Research output: Contribution to journalArticle

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