Using Single-Cell Amperometry To Reveal How Cisplatin Treatment Modulates the Release of Catecholamine Transmitters during Exocytosis

Xianchan Li, Johan Dunevall, Andrew G. Ewing

Research output: Contribution to journalArticlepeer-review

Abstract

The pretreatment of cultured pheochromocytoma (PC12) cells with cis-diamminedichloroplatinum (cisplatin), an anti-cancer drug, influences the exocytotic ability of the cells in a dose-dependent manner. Low concentrations of cisplatin stimulate catecholamine release whereas high concentrations inhibit it. Single-cell amperometry reflects that 2 μm cisplatin treatment increases the frequency of exocytotic events and reduces their duration, whereas 100 μm cisplatin treatment decreases the frequency of exocytotic events and increases their duration. Furthermore, the stability of the initial fusion pore that is formed in the lipid membrane during exocytosis is also regulated differentially by different cisplatin concentrations. This study thus suggests that cisplatin influences exocytosis by multiple mechanisms.

Original languageEnglish (US)
Pages (from-to)9041-9044
Number of pages4
JournalAngewandte Chemie - International Edition
Volume55
Issue number31
DOIs
StatePublished - Jul 25 2016

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)

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