Using time-lapse fluorescence microscopy to study gene regulation

Fan Zou, Lu Bai

Research output: Contribution to journalReview article

2 Citations (Scopus)

Abstract

Time-lapse fluorescence microscopy is a powerful tool to study gene regulation. By probing fluorescent signals in single cells over extended period of time, this method can be used to study the dynamics, noise, movement, memory, inheritance, and coordination, of gene expression during cell growth, development, and differentiation. In combination with a flow-cell device, it can also measure gene regulation by external stimuli. Due to the single cell nature and the spatial/temporal capacity, this method can often provide information that is hard to get using other methods. Here, we review the standard experimental procedures and new technical developments in this field.

Original languageEnglish (US)
Pages (from-to)138-145
Number of pages8
JournalMethods
Volume159-160
DOIs
StatePublished - Apr 15 2019

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Fluorescence microscopy
Fluorescence Microscopy
Gene expression
Genes
Cell growth
Growth and Development
Noise
Data storage equipment
Gene Expression
Equipment and Supplies

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

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Using time-lapse fluorescence microscopy to study gene regulation. / Zou, Fan; Bai, Lu.

In: Methods, Vol. 159-160, 15.04.2019, p. 138-145.

Research output: Contribution to journalReview article

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