Uterine oestrogen and progesterone receptors in the ovariectomized rat

A. Manni, R. Baker, B. M. Arafah, O. H. Pearson

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The effect was studied of repeated injections of oestradiol-17β (5, 10, 25, 50 μg) given for various lengths of time (3, 5, 9 days) on total cell content of oestrogen receptors and cytosol progesterone receptors in the uteri of ovariectomized rats. An additional group of rats was injected daily with 50μg oestradiol benzoate (OB) for 9 days in order to achieve a more sustained concentration of oestradiol in the blood. Injections were begun 24 h after ovariectomy and the rats were killed 24 h after the last injection. Daily administration of 5 μg oestradiol prevented the initial transient rise in oestrogen receptors which was observed in the uteri of untreated rats after ovariectomy. Repeated injections of 10μg oestrogen produced an initial lowering in oestrogen receptors after 3 days of treatment which was followed by a prompt rise at 5 and 9 days when treatment was continued. A significant reduction in oestrogen receptors occurred at all times studied when rats were injected daily with 25 and 50 μg oestradiol. A more profound reduction in oestrogen receptors was observed in the group of rats treated for 9 days with 50 μg OB. Synthesis of progesterone receptors was stimulated by all doses of oestrogen studied. Concentrations of progesterone receptors were significantly higher after 3 and 5 days of treatment with 25 and 50 μg oestrogen. After 9 days of treatment, however, concentrations of progesterone receptors were virtually identical in all treated groups, including the group treated with OB. We have concluded that large doses of oestrogen significantly decrease oestrogen receptor content in the rat uterus, especially when OB is used. The degree of reduction, however, is only moderate under these experimental conditions and is insufficient to inhibit synthesis of progesterone receptors.

Original languageEnglish (US)
Pages (from-to)281-287
Number of pages7
JournalJournal of Endocrinology
Volume91
Issue number2
DOIs
StatePublished - Jan 1 1981

Fingerprint

Progesterone Receptors
Estrogen Receptors
Estradiol
Estrogens
Uterus
Injections
Ovariectomy
Therapeutics
Cytosol
estradiol 3-benzoate

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Manni, A. ; Baker, R. ; Arafah, B. M. ; Pearson, O. H. / Uterine oestrogen and progesterone receptors in the ovariectomized rat. In: Journal of Endocrinology. 1981 ; Vol. 91, No. 2. pp. 281-287.
@article{d028e29c38a04a019d975d3a3c908b07,
title = "Uterine oestrogen and progesterone receptors in the ovariectomized rat",
abstract = "The effect was studied of repeated injections of oestradiol-17β (5, 10, 25, 50 μg) given for various lengths of time (3, 5, 9 days) on total cell content of oestrogen receptors and cytosol progesterone receptors in the uteri of ovariectomized rats. An additional group of rats was injected daily with 50μg oestradiol benzoate (OB) for 9 days in order to achieve a more sustained concentration of oestradiol in the blood. Injections were begun 24 h after ovariectomy and the rats were killed 24 h after the last injection. Daily administration of 5 μg oestradiol prevented the initial transient rise in oestrogen receptors which was observed in the uteri of untreated rats after ovariectomy. Repeated injections of 10μg oestrogen produced an initial lowering in oestrogen receptors after 3 days of treatment which was followed by a prompt rise at 5 and 9 days when treatment was continued. A significant reduction in oestrogen receptors occurred at all times studied when rats were injected daily with 25 and 50 μg oestradiol. A more profound reduction in oestrogen receptors was observed in the group of rats treated for 9 days with 50 μg OB. Synthesis of progesterone receptors was stimulated by all doses of oestrogen studied. Concentrations of progesterone receptors were significantly higher after 3 and 5 days of treatment with 25 and 50 μg oestrogen. After 9 days of treatment, however, concentrations of progesterone receptors were virtually identical in all treated groups, including the group treated with OB. We have concluded that large doses of oestrogen significantly decrease oestrogen receptor content in the rat uterus, especially when OB is used. The degree of reduction, however, is only moderate under these experimental conditions and is insufficient to inhibit synthesis of progesterone receptors.",
author = "A. Manni and R. Baker and Arafah, {B. M.} and Pearson, {O. H.}",
year = "1981",
month = "1",
day = "1",
doi = "10.1677/joe.0.0910281",
language = "English (US)",
volume = "91",
pages = "281--287",
journal = "Journal of Endocrinology",
issn = "0022-0795",
publisher = "Society for Endocrinology",
number = "2",

}

Uterine oestrogen and progesterone receptors in the ovariectomized rat. / Manni, A.; Baker, R.; Arafah, B. M.; Pearson, O. H.

In: Journal of Endocrinology, Vol. 91, No. 2, 01.01.1981, p. 281-287.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Uterine oestrogen and progesterone receptors in the ovariectomized rat

AU - Manni, A.

AU - Baker, R.

AU - Arafah, B. M.

AU - Pearson, O. H.

PY - 1981/1/1

Y1 - 1981/1/1

N2 - The effect was studied of repeated injections of oestradiol-17β (5, 10, 25, 50 μg) given for various lengths of time (3, 5, 9 days) on total cell content of oestrogen receptors and cytosol progesterone receptors in the uteri of ovariectomized rats. An additional group of rats was injected daily with 50μg oestradiol benzoate (OB) for 9 days in order to achieve a more sustained concentration of oestradiol in the blood. Injections were begun 24 h after ovariectomy and the rats were killed 24 h after the last injection. Daily administration of 5 μg oestradiol prevented the initial transient rise in oestrogen receptors which was observed in the uteri of untreated rats after ovariectomy. Repeated injections of 10μg oestrogen produced an initial lowering in oestrogen receptors after 3 days of treatment which was followed by a prompt rise at 5 and 9 days when treatment was continued. A significant reduction in oestrogen receptors occurred at all times studied when rats were injected daily with 25 and 50 μg oestradiol. A more profound reduction in oestrogen receptors was observed in the group of rats treated for 9 days with 50 μg OB. Synthesis of progesterone receptors was stimulated by all doses of oestrogen studied. Concentrations of progesterone receptors were significantly higher after 3 and 5 days of treatment with 25 and 50 μg oestrogen. After 9 days of treatment, however, concentrations of progesterone receptors were virtually identical in all treated groups, including the group treated with OB. We have concluded that large doses of oestrogen significantly decrease oestrogen receptor content in the rat uterus, especially when OB is used. The degree of reduction, however, is only moderate under these experimental conditions and is insufficient to inhibit synthesis of progesterone receptors.

AB - The effect was studied of repeated injections of oestradiol-17β (5, 10, 25, 50 μg) given for various lengths of time (3, 5, 9 days) on total cell content of oestrogen receptors and cytosol progesterone receptors in the uteri of ovariectomized rats. An additional group of rats was injected daily with 50μg oestradiol benzoate (OB) for 9 days in order to achieve a more sustained concentration of oestradiol in the blood. Injections were begun 24 h after ovariectomy and the rats were killed 24 h after the last injection. Daily administration of 5 μg oestradiol prevented the initial transient rise in oestrogen receptors which was observed in the uteri of untreated rats after ovariectomy. Repeated injections of 10μg oestrogen produced an initial lowering in oestrogen receptors after 3 days of treatment which was followed by a prompt rise at 5 and 9 days when treatment was continued. A significant reduction in oestrogen receptors occurred at all times studied when rats were injected daily with 25 and 50 μg oestradiol. A more profound reduction in oestrogen receptors was observed in the group of rats treated for 9 days with 50 μg OB. Synthesis of progesterone receptors was stimulated by all doses of oestrogen studied. Concentrations of progesterone receptors were significantly higher after 3 and 5 days of treatment with 25 and 50 μg oestrogen. After 9 days of treatment, however, concentrations of progesterone receptors were virtually identical in all treated groups, including the group treated with OB. We have concluded that large doses of oestrogen significantly decrease oestrogen receptor content in the rat uterus, especially when OB is used. The degree of reduction, however, is only moderate under these experimental conditions and is insufficient to inhibit synthesis of progesterone receptors.

UR - http://www.scopus.com/inward/record.url?scp=0019780210&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019780210&partnerID=8YFLogxK

U2 - 10.1677/joe.0.0910281

DO - 10.1677/joe.0.0910281

M3 - Article

C2 - 7299327

AN - SCOPUS:0019780210

VL - 91

SP - 281

EP - 287

JO - Journal of Endocrinology

JF - Journal of Endocrinology

SN - 0022-0795

IS - 2

ER -