Utilization of γ-32P-GTP by ectoprotein kinase of 3T3 and SV40 3T3 cells

Andrea Marie Mastro, Marion J. Sniezek

Research output: Contribution to journalArticle

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Abstract

The ectoprotein membrane kinase(s) of 3T3 and SV40 3T3 cells were found to use GTP as a phosphate donor. Phosphorylation with GTP was similar to that found with ATP, previously used to identify the kinase activity in this system (Mastro and Rozengurt, 1976). Under the same conditions, phosphorylation was greater with ATP than GTP. However, with either nucleotide the phosphorylation was higher in SV40 transformed and growing 3T3 cells than in quiescent ones. Using SDS polyacrylamide gel electrophoresis and radioautography we found that similar substrates were phosphorylated with GTP or ATP.

Original languageEnglish (US)
Pages (from-to)385-392
Number of pages8
JournalCell Biology International Reports
Volume6
Issue number4
DOIs
StatePublished - Jan 1 1982

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3T3 Cells
Guanosine Triphosphate
Adenosine Triphosphate
Phosphorylation
Autoradiography
Polyacrylamide Gel Electrophoresis
Phosphotransferases
Nucleotides
Phosphates
Membranes
ectoprotein kinase

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Mastro, Andrea Marie ; Sniezek, Marion J. / Utilization of γ-32P-GTP by ectoprotein kinase of 3T3 and SV40 3T3 cells. In: Cell Biology International Reports. 1982 ; Vol. 6, No. 4. pp. 385-392.
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Utilization of γ-32P-GTP by ectoprotein kinase of 3T3 and SV40 3T3 cells. / Mastro, Andrea Marie; Sniezek, Marion J.

In: Cell Biology International Reports, Vol. 6, No. 4, 01.01.1982, p. 385-392.

Research output: Contribution to journalArticle

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