Utilization of carbon substrates, electrophoretic enzyme patterns, and symbiotic performance of plasmid-cured clover rhizobia

J. I. Baldani, R. W. Weaver, M. F. Hynes, Bertrand D. Eardly

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Plasmids in Rhizobium spp. are relatively large, numerous, and difficult to cure. Except for the symbiotic plasmid, little is known about their functions. The primary objective of our investigation was to obtain plasmid- cured derivatives of Rhizobium leguminosarum bv. trifolii by using a direct selection system and to determine changes in the phenotype of the cured strains. Three strains of rhizobia were utilized that contained three, four, and five plasmids. Phenotypic effects observed after curing of plasmids indicated that the plasmids were involved in the utilization of adonitol, arabinose, catechol, glycerol, inositol, lactose, malate, rhamnose, and sorbitol and also influenced motility, lipopolysaccharide production, and utilization of nitrate. Specific staining of 26 enzymes electrophoretically separated on starch gels indicated that superoxide dismutase, hexokinase, and carbamate kinase activities were affected by curing of plasmids. Curing of cryptic plasmids also influenced nodulation and growth of plants on nitrogen- deficient media. The alteration in the ability to utilize various substrates after curing of plasmids suggests that the plasmids may encode genes that contribute significantly to the saprophytic competence of rhizobia in soil.

Original languageEnglish (US)
Pages (from-to)2308-2314
Number of pages7
JournalApplied and environmental microbiology
Volume58
Issue number7
StatePublished - Jan 1 1992

Fingerprint

Medicago
Rhizobium
enzyme substrates
rhizobacterium
plasmid
plasmids
Plasmids
Carbon
plasmid curing
enzyme
substrate
carbon
Enzymes
carbamate kinase
Rhizobium leguminosarum bv. trifolii
hexokinase
catechol
rhamnose
starch gels
carbamates

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

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Utilization of carbon substrates, electrophoretic enzyme patterns, and symbiotic performance of plasmid-cured clover rhizobia. / Baldani, J. I.; Weaver, R. W.; Hynes, M. F.; Eardly, Bertrand D.

In: Applied and environmental microbiology, Vol. 58, No. 7, 01.01.1992, p. 2308-2314.

Research output: Contribution to journalArticle

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