Utilization of glucose-based medium and optimization of Bacillus subtilis natto growth parameters for vitamin K (menaquinone-7) production in biofilm reactors

Ehsan Mahdinia, Ali Demirci, Aydin Berenjian

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Menaquinone-7 (MK-7) is the most potent form of vitamin K prescribed as a dietary supplement. MK-7 is commonly produced via static fermentation of Bacillus subtilis natto. The fermentation of this bacterium is associated with formation of large amounts of pellicles and biofilm, which are effective in MK-7 production, but also result in significant heat and mass transfer challenges during the scale-up process. Thus, the objectives of this study were to develop and evaluate the possibility of using a biofilm reactor for MK-7 production. In this research, biofilm reactors were constructed using the Plastic Composite Supports (PCS). In order to optimize the fermentation parameters for MK-7 production, Central Composite Design (CCD) was carried out along with supplementary runs to determine the optimum temperature, pH, and agitation. The biofilm reactors were able to produce a maximum concentration of 18.45 ± 0.76 mg/L of MK-7, which was 237% higher than the suspended-cell fermentation. Therefore, the present work suggests the possibility of using biofilm reactors as a new and effective fermentation strategy to address the issues associated with MK-7 fermentation.

Original languageEnglish (US)
Pages (from-to)219-224
Number of pages6
JournalBiocatalysis and Agricultural Biotechnology
Volume13
DOIs
StatePublished - Jan 1 2018

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Agronomy and Crop Science

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