UV cross-linking of DNA-protein complexes in vitro

Research output: Contribution to journalArticle

Abstract

UV cross-linking provides a powerful method to identify proteins that interact with DNA. By using DNA fragments that have been selectively radiolabeled in specific regions of the fragment, it is possible to detect the contacts of specific components in relatively complex mixtures of proteins. Moreover, as many of the functions of DNA are performed by large complexes composed of several multi-subunit proteins, this highly specific strategy of cross-linking can be used to determine the locations of specific polypeptides on the DNA.

Original languageEnglish (US)
Pages (from-to)162-168
Number of pages7
JournalMethods in Molecular and Cellular Biology
Volume5
Issue number3
StatePublished - 1995

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DNA
Proteins
Protein Subunits
Complex Mixtures
Peptides
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Clinical Biochemistry
  • Molecular Biology

Cite this

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abstract = "UV cross-linking provides a powerful method to identify proteins that interact with DNA. By using DNA fragments that have been selectively radiolabeled in specific regions of the fragment, it is possible to detect the contacts of specific components in relatively complex mixtures of proteins. Moreover, as many of the functions of DNA are performed by large complexes composed of several multi-subunit proteins, this highly specific strategy of cross-linking can be used to determine the locations of specific polypeptides on the DNA.",
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UV cross-linking of DNA-protein complexes in vitro. / Gilmour, David Scott.

In: Methods in Molecular and Cellular Biology, Vol. 5, No. 3, 1995, p. 162-168.

Research output: Contribution to journalArticle

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