Vaccination with lipopolysaccharide isolated from the live vaccine strain of Francisella tularensis protects against murine tularemia

A. H. Fortier, R. B. Narayanan, Joseph Drabick, J. C. Williams, C. A. Nacy

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Abstract

A monoclonal antibody (mAb) prepared against the lipopolysaccharide (LPS) of Francisella tularensis was inoculated into mice 2 h prior to challenge with 1000 LD50 F. tularensis, the live vaccine strain (LVS). Mice treated with 2-mg mAb survived the challenge inoculum. Successful passive transfer of protection by the anti-LPS monoclonal led us to explore the active vaccine potential of LPS extracted from F. tularensis strain LVS. Mice immunized with LVS-LPS generated Ab that recognized phenol water extracted LPS from Francisella, as well as whole F. tularensis LVS bacteria. Ab did not crossreact with Escherichia coli, Yersinia enterocolitica, or Brucella abortus, but did react with the virulent SCHU-4 strain of F. tularensis. LVS-LPS immunized mice were protected from challenge with 103 LD50 F tularensis LVS inoculated intraperitoneally and 107 F. tularensis LVS inoculated intradermally, but were not protected against lethal challenge with an unrelated gram-negative organism, E. coli BORT.

Original languageEnglish (US)
Pages (from-to)193-202
Number of pages10
JournalVaccine Research
Volume5
Issue number4
StatePublished - Dec 1 1996

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All Science Journal Classification (ASJC) codes

  • Immunology
  • Microbiology (medical)

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